Initial Studies on Isolation and Characterization of Antioxidant Compounds from Immature Berries of Ampelopsis grandulosa

Vera Fitriya Ersalena, H. Miyazaki, Kosaku Takahashi, K. Hara, H. Matsuura
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引用次数: 1

Abstract

This initial study is aimed to isolate and characterize the antioxidant compounds from immature berries of Ampelopsis grandulosa (Vitaceae). In Japan, A. grandulosa is utilized for folk medicine because some local people in Japan believe that it has beneficial effects to human’ health. However, the studies of bioactive compounds in particular antioxidant activity has not been reported for A. grandulosa. The antioxidant activities were monitored using 2,2-dyphenyl-1-perylhydrazyl (DPPH) method. The chemical structures of active compounds were determined using spectroscopic techniques such as GC-MS, H NMR, C/DEPT NMR, COSY, HMQC, and HMBC. A mixture of fatty acids containing palmitic acid, stealic acid, oleic acid, palmitoleic acid, trans-palmitoleic acid, and linoleic acid exhibit the radical scavenging activity. In addition, (+)-catechin and ethyl gallate have been also isolated as powerful antioxidant. Keywords—Antioxidant compounds, DPPH, radical scavenging, Ampelopsis grandulosa.
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小葡萄未成熟果实中抗氧化化合物的分离及特性初步研究
本初步研究旨在分离和表征葡萄科葡萄属葡萄未成熟果实中的抗氧化化合物。在日本,黄花仙子被用于民间医药,因为一些日本当地人相信它对人体健康有益。然而,对其生物活性化合物特别是抗氧化活性的研究尚未见报道。采用2,2-dyphenyl-1-perylhydrazyl (DPPH)法测定其抗氧化活性。采用GC-MS、H NMR、C/DEPT NMR、COSY、HMQC和HMBC等光谱技术测定活性化合物的化学结构。含有棕榈酸、隐身酸、油酸、棕榈油酸、反式棕榈油酸和亚油酸的脂肪酸混合物具有清除自由基的活性。此外,(+)-儿茶素和没食子酸乙酯也被分离出来作为强抗氧化剂。关键词:抗氧化化合物;DPPH;自由基清除;
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