Influence of two electroejaculation voltages on the seminal quality of alpacas (Vicugna pacos)

Abstract

The objective of the research was to evaluate the influence of two voltages in the electroejaculation protocols on the seminal quality of alpacas ( Vicugna pacos ). t was developed in the Reproductive Biotechnology Laboratory, Salache Academic Experimental Center, Technical University of Cotopaxi. Eight male alpacas were used and from each one two ejaculates were collected weekly, one using the electroejaculation protocol that used several 10 volt stimuli, and the other using a protocol where the stimuli were 12 volts. The volume of the ejaculated determined itself for direct observation in measuring glass, la concentration; sperm motility, viability and morphology by Neubauer chamber, DM4B YDM6B mass microscope at 10X, Eosin-Nigrosin staining and observation of semen-eosin staining, respectively. Volume, concentration of ejaculates, motility, seminal pathologies were compared according to the electroejaculation protocol by means of the Student's t test for independent samples. The volume of the ejaculates was 0.75±0.20 and 1.10±0.39 mL, the concentration 2.12±1.93 and 2.25±1.89 (x10 6 ), the sperm mass motility 81. 25±11.81 % and 80.00±14.14 %, mortality 17.50±9.57 and 15.00±10.80 % and morphoanomalies 12.50±6.45 and 13.50±8.01 % in the animals that received electroejaculation with 10 and 12 volts, respectively. There were no significant differences (p> 0.05) in the seminal parameters evaluated according to the voltage used in the ejaculation protocol. It is concluded that the increase in voltage in the electroejaculation protocol had no influence on the seminal quality parameters of alpacas