Noninvasive Biomarkers for Alcohol-Related Liver Disease-A Proteomic Related Preliminary Report.

IF 1.5 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Indian Journal of Clinical Biochemistry Pub Date : 2024-07-01 Epub Date: 2023-03-03 DOI:10.1007/s12291-023-01120-9

Prabhudas Nelaturi, Sangeetha P Kademani, Vithiavathi Siva Subramanian, Sambandam Ravikumar

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引用次数: 0

Abstract

Increased alcohol intake over decades leads to progressive alcohol-related liver disease (ALD) and contributes to increased mortality. It is characterized by reduced platelet count. Platelets have a role in protecting vascular integrity and involved in liver regeneration. Alcohol affects the platelet count and its function. Platelet function is regulated by their proteins, released during pathophysiological conditions. Therefore, platelet proteome plays a vital role during ALD. This preliminary study consists of 10 patients with ALD. It includes the preparation of human platelets for the proteomic approach. We performed liquid chromatography-mass spectrometry for the samples. A total of 536 proteins were identified in patients with ALD of which 31 proteins were mentioned as a candidate based on their clinical significance. The advancement of diagnostic or therapeutic tools based on the application of platelet proteins in ALD is still far off. Platform for platelet and its proteome research may give diagnostic and prognostic insights into ALD. Platelet proteomes could possibly be concluded as therapeutic and potential diagnostic or prognostic markers in ALD.

Supplementary information: The online version contains supplementary material available at 10.1007/s12291-023-01120-9.

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酒精相关肝病的无创生物标志物--蛋白质组学相关初步报告

几十年来，酒精摄入量的增加导致酒精相关肝病（ALD）的进展，并导致死亡率上升。这种疾病的特点是血小板数量减少。血小板具有保护血管完整性和参与肝脏再生的作用。酒精会影响血小板数量及其功能。血小板的功能受其蛋白质的调节，在病理生理条件下释放。因此，血小板蛋白质组在 ALD 期间起着至关重要的作用。这项初步研究包括 10 名 ALD 患者。它包括为蛋白质组学方法制备人体血小板。我们对样本进行了液相色谱-质谱分析。在 ALD 患者体内共鉴定出 536 种蛋白质，其中 31 种蛋白质因其临床意义而被列为候选蛋白质。基于血小板蛋白在 ALD 中的应用而开发诊断或治疗工具还很遥远。血小板及其蛋白质组研究平台可为 ALD 的诊断和预后提供见解。血小板蛋白质组可能被认为是ALD的治疗和潜在诊断或预后标志物：在线版本包含补充材料，可查阅 10.1007/s12291-023-01120-9。

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来源期刊

Indian Journal of Clinical Biochemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

4.50

自引率

4.80%

发文量

期刊介绍： The primary mission of the journal is to promote improvement in the health and well-being of community through the development and practice of clinical biochemistry and dissemination of knowledge and recent advances in this discipline among professionals, diagnostics industry, government and non-government organizations. Indian Journal of Clinical Biochemistry (IJCB) publishes peer reviewed articles that contribute to the existing knowledge in all fields of Clinical biochemistry, either experimental or theoretical, particularly deal with the applications of biochemistry, molecular biology, genetics, biotechnology, and immunology to the diagnosis, treatment, monitoring and prevention of human diseases. The articles published also include those covering the analytical and molecular diagnostic techniques, instrumentation, data processing, quality assurance and accreditation aspects of the clinical investigations in which chemistry has played a major role, or laboratory animal studies with biochemical and clinical relevance.