Fare Kemik İliği Hücrelerinde Siklofosfamid Tarafından İndüklenen Genotoksisiteye Karşı Tarhun (Artemisia dracunculus L.) Yaprak Ekstraktının Olası Koruyucu Etkisinin Mikronükleus Testi ile Belirlenmesi

Abstract

: This work; had been carried out by aiming the determination of the possible protective effect of the tarragon ( Artemisia dracunculus L.) leaf extract against genotoxicity of cyclophosphamide (CP), by micronucleus test. In the study, 6 test groups were formed and each group was involved 8 animals. These groups were named as: I. group is negative control group, II. group is positive control group which is formed from 50 mg/kg, i.p. CP, III. group denotes 75 mg/kg tarragon leaf extract group, IV. group denoted 150 mg/kg tarragon leaf extract group, V. group is formed from 75 mg/kg tarragon leaf extract + CP (50 mg/kg, i.p.) and VI. group is formed from 150 mg/kg tarragon leaf extract + CP (50 mg/kg, i.p.). The tarragon leaf extract was given to the mice via oral gavage during 14 days. Cyclophosphamide was injected intraperitoneally to the mice before 24 hours of the dislocation (day 15) of them. The micronucleus ratios were determined by the performing of the micronucleus tests to the bone marrow cells of dislocated mice.At the end of the study; when the statistical evaluation of micronucleus numbers were compared with the negative control group, it was determined that the MN numbers increased in the group which was exposed to CP (p<0.001) according to the negative control group. On the other hand, it was determined that the MN numbers of CP group were significantly decreased when the 75 mg/kg and 150 mg/kg doses AD in addition to the CP (p<0.001) were applied to the CP group.When an evaluation was carried out upon the PCE/NCE ratios, it was determined that values of the CP group (p<0.001) were less than values of the control group, on the other hand, when 75 mg/kg AD applied with CP (p<0.005) it was observed that the PCE/NCE ratios were decreased compared to the situation that applied 150 mg/kg AD to the CP group (p<0.001).As a result; it was determined that 75 mg/kg and 150 mg/kg doses of Artemisia dracunculus L. leaf extract showed protective effect in mouse bone marrow cells by leading to decrease of the increase in micronucleus which was caused by cyclophosphamide.