Attachment of 80S ribosome to lipid monolayers at air/water interface: An electron microscopy study†

Abstract

The adsorption of 80S ribosome from rat liver to the surface of lipid monolayers at the air/water interface was examined by electron microscopy (EM) using a negative staining method. The results showed that, a large number of 80S ribosomes can be adsorbed to the lipid monolayers containing positively charged octadecylamine (SA), whereas the adsorption of ribosomes to the surface of neutral or negatively charged lipid monolayers was negligible. There existed a proper ratio of SA to complemented neutral lipids which facilitated the maximum binding of ribosomes. Increasing the subphase pH value will enhance the adsorption of ribosome, but when raising the subphase concentrations of K⁺, Mg²⁺ and glycerol, the adsorption of ribosomes can be weakened, suggesting that the driving forces of the adsorption mainly come from the electrostatic interactions between the ribosome and the lipids. The important characteristics of such interactions between the 80S rat liver ribosomes and the lipid membranes, as revealed by this new technology, which may help in the further understanding of the protein biosynthesis is discussed.