The combination of Neosartorya (Aspergillus) fischeri antifungal proteins with rationally designed γ-core peptide derivatives is effective for plant and crop protection.
Liliána Tóth, Péter Poór, Attila Ördög, Györgyi Váradi, Attila Farkas, Csaba Papp, Gábor Bende, Gábor K Tóth, Gábor Rákhely, Florentine Marx, László Galgóczy
{"title":"The combination of <i>Neosartorya</i> (<i>Aspergillus</i>) <i>fischeri</i> antifungal proteins with rationally designed γ-core peptide derivatives is effective for plant and crop protection.","authors":"Liliána Tóth, Péter Poór, Attila Ördög, Györgyi Váradi, Attila Farkas, Csaba Papp, Gábor Bende, Gábor K Tóth, Gábor Rákhely, Florentine Marx, László Galgóczy","doi":"10.1007/s10526-022-10132-y","DOIUrl":null,"url":null,"abstract":"<p><p>Plant pathogenic fungi are responsible for enormous crop losses worldwide. Overcoming this problem is challenging as these fungi can be highly resistant to approved chemical fungicides. There is thus a need to develop and introduce fundamentally new plant and crop protection strategies for sustainable agricultural production. Highly stable extracellular antifungal proteins (AFPs) and their rationally designed peptide derivatives (PDs) constitute feasible options to meet this challenge. In the present study, their potential for topical application to protect plants and crops as combinatorial biofungicides is supported by the investigation of two <i>Neosartorya</i> (<i>Aspergillus</i>) <i>fischeri</i> AFPs (NFAP and NFAP2) and their γ-core PDs. Previously, the biofungicidal potential of NFAP, its rationally designed γ-core PD (γ<sup>NFAP</sup>-opt), and NFAP2 was reported. Susceptibility tests in the present study extended the in vitro antifungal spectrum of NFAP2 and its γ-core PD (γ<sup>NFAP2</sup>-opt) to <i>Botrytis</i>, <i>Cladosporium</i>, and <i>Fusarium</i> spp. Besides, in vitro additive or indifferent interactions, and synergism were observed when NFAP or NFAP2 was applied in combination with γ<sup>NFAP</sup>-opt. Except for γ<sup>NFAP2</sup>-opt, the investigated proteins and peptides did not show any toxicity to tomato plant leaves. The application of NFAP in combination with γ<sup>NFAP</sup>-opt effectively inhibited conidial germination, biofilm formation, and hyphal extension of the necrotrophic mold <i>Botrytis cinerea</i> on tomato plant leaves. However, the same combination only partially impeded the <i>B. cinerea</i>-mediated decay of tomato fruits, but mitigated the symptoms. Our results highlight the feasibility of using the combination of AFP and PD as biofungicide for the fungal infection control in plants and crops.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s10526-022-10132-y.</p>","PeriodicalId":8878,"journal":{"name":"BioControl","volume":"15 1","pages":"249-262"},"PeriodicalIF":2.7000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8993730/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BioControl","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s10526-022-10132-y","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/2/4 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Plant pathogenic fungi are responsible for enormous crop losses worldwide. Overcoming this problem is challenging as these fungi can be highly resistant to approved chemical fungicides. There is thus a need to develop and introduce fundamentally new plant and crop protection strategies for sustainable agricultural production. Highly stable extracellular antifungal proteins (AFPs) and their rationally designed peptide derivatives (PDs) constitute feasible options to meet this challenge. In the present study, their potential for topical application to protect plants and crops as combinatorial biofungicides is supported by the investigation of two Neosartorya (Aspergillus) fischeri AFPs (NFAP and NFAP2) and their γ-core PDs. Previously, the biofungicidal potential of NFAP, its rationally designed γ-core PD (γNFAP-opt), and NFAP2 was reported. Susceptibility tests in the present study extended the in vitro antifungal spectrum of NFAP2 and its γ-core PD (γNFAP2-opt) to Botrytis, Cladosporium, and Fusarium spp. Besides, in vitro additive or indifferent interactions, and synergism were observed when NFAP or NFAP2 was applied in combination with γNFAP-opt. Except for γNFAP2-opt, the investigated proteins and peptides did not show any toxicity to tomato plant leaves. The application of NFAP in combination with γNFAP-opt effectively inhibited conidial germination, biofilm formation, and hyphal extension of the necrotrophic mold Botrytis cinerea on tomato plant leaves. However, the same combination only partially impeded the B. cinerea-mediated decay of tomato fruits, but mitigated the symptoms. Our results highlight the feasibility of using the combination of AFP and PD as biofungicide for the fungal infection control in plants and crops.
Supplementary information: The online version contains supplementary material available at 10.1007/s10526-022-10132-y.
期刊介绍：
BioControl, the official journal of the International Organization for Biological Control, presents original papers on basic and applied research in all aspects of biological control of invertebrate, vertebrate and weed pests, and plant diseases. Coverage includes biology and ecology of organisms for biological control, and aspects of use including biological controls for integrated pest management, such as plant resistance, pheromones and intercropping. Papers presenting only laboratory trials on non-target effects of pesticides on natural enemies are not considered but papers dealing with the corresponding effects on community dynamics of natural enemies are welcomed.
Organisms covered include parasitoids, invertebrate and vertebrate predators of pest animals and plants, mites, plant and insect pathogens, nematodes, and weeds.
The journal publishes interdisciplinary papers with a global perspective on the use of biological control in integrated pest management systems, and related developments in molecular biology and biotechnology that have direct relevance.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
