{"title":"Spectrophotometric Assay for Diaminopimelate Decarboxylase of Higher Plants using Pea Diamine Oxidase","authors":"Yonezo Suzuki , Manami Yoshishige","doi":"10.1016/S0015-3796(11)80002-8","DOIUrl":null,"url":null,"abstract":"<div><p>A sensitive and comparatively simple spectrophotometric method for the assay of <em>meso</em>-diaminopimelic acid decarboxylase is presented. It is based upon the coupled reaction of the decarboxylase with pea diamine oxidase and horseradish peroxidase.L-Lysine, a product of <em>meso</em>-diaminopimelic acid decarboxylase is oxidized by pea diamine oxidase and the hydrogen peroxide generated is estimated as the red colour (quinoneimine dye) formed on the peroxidative oxidation of the mixture of 3,5-dichloro-2-hydroxybenzenesulfonate and 4-aminoantipyrine. The method was compared with the result by a high performance liquid chromatography assay.</p></div>","PeriodicalId":8798,"journal":{"name":"Biochemie und Physiologie der Pflanzen","volume":"188 3","pages":"Pages 153-159"},"PeriodicalIF":0.0000,"publicationDate":"1992-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0015-3796(11)80002-8","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemie und Physiologie der Pflanzen","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0015379611800028","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
A sensitive and comparatively simple spectrophotometric method for the assay of meso-diaminopimelic acid decarboxylase is presented. It is based upon the coupled reaction of the decarboxylase with pea diamine oxidase and horseradish peroxidase.L-Lysine, a product of meso-diaminopimelic acid decarboxylase is oxidized by pea diamine oxidase and the hydrogen peroxide generated is estimated as the red colour (quinoneimine dye) formed on the peroxidative oxidation of the mixture of 3,5-dichloro-2-hydroxybenzenesulfonate and 4-aminoantipyrine. The method was compared with the result by a high performance liquid chromatography assay.
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