In vitro Evaluation of Mesoporous Carbonated Hydroxyapatite in MC3T3-E1 Osteoblast Cells

Procedia Chemistry Pub Date : 2016-01-01 DOI:10.1016/j.proche.2016.03.103

Nur Farahiyah Mohammad , Radzali Othman , Nurul Asma Abdullah , Fei Yee Yeoh

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引用次数: 21

Abstract

Nanoporous bioceramic recently has gained attention as a drug storage and release host in the therapeutic application. Mesoporous carbonated hydroxyapatite (MCHA) is a nanoporous bioceramic with pore sized ranged between 2-50 nm. In order to use in drug delivery application, this material should demonstrate acceptable cytotoxic activity and osteoinductive response. Therefore, this study aimed to evaluate the effect of mesoporous carbonated hydroxyapatite on viability and alkaline phosphatase (ALP) activity of MC3T3-E1 osteoblasts cell line. These parameters represent cytotoxicity level and osteinductivity capability of the materials. MC3T3-E1 cells were cultured in 25 mg/ml extraction of mesoporous carbonated hydroxyapatite (meso-CHA) and non-porous CHA (np-CHA) for up to 7 days for viability and 14 days for ALP test. Results indicate that meso-CHA shows better cytotoxicity properties compare to np-CHA. Intracellular ALP of cells treated with meso-CHA was higher than np-CHA.

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MC3T3-E1成骨细胞中介孔碳化羟基磷灰石的体外研究

近年来，纳米多孔生物陶瓷作为药物储存和释放载体在治疗领域的应用日益受到关注。介孔碳化羟基磷灰石(MCHA)是一种孔径在2 ~ 50nm之间的纳米多孔生物陶瓷。为了在给药应用中使用，该材料应表现出可接受的细胞毒活性和骨诱导反应。因此，本研究旨在评价中孔碳化羟基磷灰石对MC3T3-E1成骨细胞活力和碱性磷酸酶(ALP)活性的影响。这些参数反映了材料的细胞毒性水平和成骨能力。MC3T3-E1细胞在25 mg/ml的中孔碳化羟基磷灰石(meso-CHA)和无孔CHA (np-CHA)提取物中培养7天，ALP检测14天。结果表明，与np-CHA相比，meso-CHA具有更好的细胞毒性。中位- cha处理的细胞内ALP高于np-CHA。

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Procedia Chemistry

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