Interactome analysis reveals molecular mechanisms underlying the association between selenium binding protein 1 expression and the malignant features of tumor cells

Takashi Tajima, F. Kito, T. Ohta, K. Shiozawa, A. Kawai, T. Kondo
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Abstract

The potential biological and clinical significance of selenium binding protein 1 (SBP1) has been suggested in various types of cancer. To evaluate the role of SBP1 and reveal the molecular basis for its function, we examined the SBP1 protein complex. A gene transfection assay revealed that overexpression of SBP1 promoted proliferation and migration of A549 lung adenocarcinoma cells. Halo-tag-based affinity purification coupled with liquid chromatography-tandem mass spectrometry identified 23 components of the SBP1 protein complex. The functional classification of these 23 proteins suggests that the SBP1 complex participates in critical biological events including cell structure, protein translation, stress response, chaperone, and apoptosis. Moreover, the SBP1 complex includes several proteins that are aberrantly expressed in cancers. These finding indicate that SBP1 may function coordinately with these multiple proteins to facilitate cancer progression. A comprehensive study of the multiple proteins associated with SPB1 together with an examination of individual proteins will be required to elucidate the roles of aberrant SBP1 regulation in cancer progression.
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相互作用组分析揭示了硒结合蛋白1表达与肿瘤细胞恶性特征相关的分子机制
硒结合蛋白1 (SBP1)的潜在生物学和临床意义已被提出在各种类型的癌症。为了评估SBP1的作用并揭示其功能的分子基础,我们检测了SBP1蛋白复合物。基因转染实验显示,SBP1的过表达促进了A549肺腺癌细胞的增殖和迁移。基于halo标签的亲和纯化结合液相色谱-串联质谱法鉴定了SBP1蛋白复合物的23个组分。这23种蛋白的功能分类表明,SBP1复合物参与了包括细胞结构、蛋白质翻译、应激反应、伴侣和凋亡在内的关键生物学事件。此外,SBP1复合体包括几种在癌症中异常表达的蛋白质。这些发现表明SBP1可能与这些多种蛋白协同作用,促进癌症进展。需要对与SPB1相关的多种蛋白进行全面研究,并对单个蛋白进行检查,以阐明SBP1异常调节在癌症进展中的作用。
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