Investigation on Lipopolysaccharide Activated Microglia by Phosphoproteomics and Phosphoinositide Lipidomics

IF 0.4 Q4 SPECTROSCOPY Mass Spectrometry Letters Pub Date : 2014-09-30 DOI:10.5478/MSL.2014.5.3.70

Young Jun Kim, Hackyoung Kim, Kwangmo Noh

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Abstract

Abstract: Microglia are the confined immune cells of the central nervous system (CNS). In response to injury or infection,microglia readily become activated and release proinflammatory mediators that are believed to contribute to microglia-mediatedneurodegeneration. In the present study, inflammation was induced in the immortalized murine microglial cell line BV-2 bylipopolysaccharide (LPS) treatment. We firstly performed phosphoproteomics analysis and phosphoinositide lipidomics analysiswith LPS activated microglia in order to compare phosphorylation patterns in active and inactive microglia and to detect the pa t-tern of changes in phosphoinositide regulation upon activation of microglia. Mass spectrometry analysis of the phosphopro-teome of the LPS treatment group compared to that of the untreated control group revealed a notable increase in the diversity ofcellular phosphorylation upon LPS treatment. Additionally, a lipidomics analysis detected significant increases in the amounts ofphosphoinositide species in the LPS treatment. This investigation could provide an insight for understanding molecular mecha-nisms underlying microglia-mediated neurodegenerative diseases.Key words: Microglia, Lipopolysaccharide (LPS), Neuroinflammation, Phosphoproteomics, Lipidomics, Phosphoinositide

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磷脂蛋白组学和磷脂肌醇组学研究脂多糖活化的小胶质细胞

摘要:小胶质细胞是中枢神经系统(CNS)的局限性免疫细胞。在对损伤或感染的反应中，小胶质细胞很容易被激活并释放促炎介质，这些介质被认为有助于小胶质细胞介导的神经变性。本研究采用脂多糖(LPS)处理永生化小鼠小胶质细胞系BV-2诱导炎症反应。我们首先对LPS激活的小胶质细胞进行了磷酸化蛋白质组学分析和磷酸肌醇脂质组学分析，以比较活性和非活性小胶质细胞的磷酸化模式，并检测小胶质细胞激活后磷酸肌醇调节的变化规律。与未治疗对照组相比，LPS治疗组的磷酸化蛋白组的质谱分析显示，LPS治疗组的细胞磷酸化多样性显著增加。此外，脂质组学分析发现，在LPS处理中，磷酸肌苷种类的数量显著增加。这项研究为理解小胶质细胞介导的神经退行性疾病的分子机制提供了新的思路。关键词:小胶质细胞，脂多糖(LPS)，神经炎症，磷酸蛋白质组学，脂质组学，磷酸肌肽

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