In vitro drug-drug interaction studies of Gliclazide with Levofloxacin by using HPLC: guidelines for co-prescription drugs

Proceedings of 4th International Electronic Conference on Medicinal Chemistry Pub Date : 2018-10-31 DOI:10.3390/ecmc-4-05586

S. Chhajed, H. Chaudhari, Yatish R. Rajderkar, Akshada Pingle, S. Sonawane, S. Kshirsagar

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Abstract

A simple, accurate reversed-phase high-performance liquid chromatography method was developed and validated for simultaneous determination of gliclazide (GLZ) and fluoroquinolone antibacterial levofloxacin (LVO). The method was developed by using a stainless steel analytical column , C18 (250,4.6 mm,5µm). The system was operated using a mobile phase consisting of methanol and phosphate buffer (pH 3.0) at a flow rate of 0.8mL minˉ1 with ultraviolet detection monitored at wavelength 228 nm. The above method was validated using ICH analytical method validation guidelines. Utilizing HPLC techniques, an assay was intended to determine in vitro effects of levofloxacin on sulphonyl urea an anti-diabetic gliclazide. Obtained results were further verified with UV spectrophotometric method. Availability of gliclazide was reduced in the presence of levofloxacin. This in vitro analyses confirms the co-administration of gliclazide and levofloxacin and may serve for designing further in vivo studies.

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用高效液相色谱法研究格列齐特与左氧氟沙星的体外药物相互作用:共处方药物指南

建立了同时测定格列齐特(GLZ)和氟喹诺酮类抗菌药物左氧氟沙星(LVO)的简便、准确的反相高效液相色谱法。该方法采用不锈钢分析柱C18 (250,4.6 mm,5µm)。流动相为甲醇和磷酸盐缓冲液(pH 3.0)，流速为0.8mL min - 1，紫外检测波长为228 nm。上述方法采用ICH分析方法验证指南进行验证。采用高效液相色谱法测定左氧氟沙星对磺胺脲和抗糖尿病格列齐特的体外作用。用紫外分光光度法进一步验证了所得结果。在左氧氟沙星存在时，格列齐特的可用性降低。这一体外分析证实了格列齐特和左氧氟沙星的联合用药，并可能为进一步的体内研究设计服务。

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Proceedings of 4th International Electronic Conference on Medicinal Chemistry

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