Inactivation of Multidrug-Resistant Salmonella Heidelberg Isolated from Pigs by Ozonated Water under Different Conditions

Abstract

ABSTRACT Salmonella Heidelberg is frequently associated with diseases transmitted by food of animal origin, so that the cleaning and disinfection of pigs farms are important for the control of this pathogen. This research was aimed to test the biocidal potential of ozonated water in different concentrations for the inactivation of a Salmonella Heidelberg isolated from pigs. We analyzed the strain antibiotic sensitivity profile to 17 antimicrobials and exposed the strain to disinfectant and three different concentrations of ozonated water (0.5 mg/L, 2.4 mg/L, and 4.4 mg/L) for one, five, and 10 minutes, in presence and absence of organic matter (OM), and performed a quantitative assessment of the bacterial genomic DNA. Salmonella Heidelberg was characterized as multidrug-resistant. Ozonated water inhibited bacterial growth in the absence of OM after one minute, with changes in bacterial DNA concentration.