Computational tools and resources for CRISPR/Cas 9 genome editing method

Abstract

From an evolutionary point of view, archaea and bacteria have evolved to survive and blossom as communities in active environments that are, in general, stressful and unpredictable. Specifically, the abundance of viruses attacking microorganisms is a constant threat; in addition, the mutation and recombination rates in viruses make them fast-evolving predators. To sustain against such predators, archaea and bacteria have evolved to have a new multilayered defense system that has been identified as CRISPR/Cas9.1 Consequently, many labs have tapped into the potential uses for this method and active research to unravel the mechanism has paved a unique path in the development of targeted genome editing techniques.2 Among the various genome editing methods, TALENs, ZFNs, and CRISPR-Cas9 are the three foremost methods.3