Toward high performance western blotting using vacuum-driven system and laser scanner; comparison between two signal detection methods based on chemiluminescent and immunofluorescent imaging

Chisato Harada, H. Tajima, S. Hirohashi, T. Kondo
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Abstract

A vacuum-driven antibody-reaction system enables high-throughput western blotting, where the antibodies pass through protein-transferred membrane and antibody-antigen reaction is completed within 30 min. With the vacuum-based device, two protein detection methods compatible with by laser scanning were compared; one with enhanced chemiluminescence reaction and the other using fluorescence-labeled antibody. Considering sensitivity, quantitative capability, reproducibility and labor intensiveness, we concluded that fluorescence-based detection is more suitable in combination with the vacuum-driven antibody-reaction system and a laser scanner.
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利用真空驱动系统和激光扫描仪进行高效免疫印迹的研究化学发光与免疫荧光成像两种信号检测方法的比较
真空驱动的抗体反应系统实现了高通量western blotting,抗体通过蛋白转移膜,抗体-抗原反应在30分钟内完成。利用真空装置比较了两种与激光扫描兼容的蛋白质检测方法;一种是增强化学发光反应,另一种是荧光标记抗体。考虑到灵敏度、定量能力、重复性和劳动强度,我们认为基于荧光的检测更适合与真空驱动抗体反应系统和激光扫描仪相结合。
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