METABOLIC ACTIVITY AND ENERGY SUPPLY OF PERIPHERAL BLOOD LYMPHOCYTES IN RESIDENTS OF THE EUROPEAN NORTH OF RUSSIA

Abstract

Introduction: T cells are able to change their metabolism in response to activation signals. Resting T cells primarily use the oxidation of higher fatty acids and oxidative phosphorylation in mitochondria for their energy needs, whereas activated T cells switch to aerobic glycolysis and glutaminolysis, using glucose and glutamine as substrates, respectively. The aim: to determine the metabolic activity and energy supply of peripheral blood lymphocytes in practically healthy northerners by measuring the intracellular content of HIF-1, SIRT3 and ATP. Methods: 39 volunteers, residents of the Arkhangelsk region (23 women and 16 men, 23-62 years old), were examined. We determined the total number of peripheral blood lymphocytes with CD-typing of lymphocytes (CD3+, CD4+, CD8+, CD71+) by indirect immunoperoxidase reactions, the content of HIF-1 and SIRT3 in the lymphocyte lysate using enzyme immunoassay, the concentration of ATP using the luciferin-luciferase reaction. Statistical analysis was carried out in "Statistica 10.0", cluster analysis was applied using the "K means" method. Mean values ​​(M) and standard deviations (SD) were calculated, the normal distribution was tested by the Kolmogorov-Smirnov and Lilliefors criterion. Student's t-test was calculated and the differences were considered statistically significant at p 0.05. Results: it was found that in the examined volunteers, the metabolic activity of lymphocytes associated with HIF-1 regulation differs statistically significantly, while in the group with a lower total number of lymphocytes and their phenotypes (CD3+, CD4+, CD8+, CD71+) there is a predominant glycolytic orientation of metabolism and a higher level of cells energy supply. Conclusion: metabolic activity, which can be judged by the HIF-1/SIRT3 ratio, has a significant impact on the differentiation, proliferation, functioning, and fate of T cells.