Expression of the genes (CBF2, CBF4, NCED1, MYB14, PLD) during acclimation and de-acclimation in dormant buds of Chambourcin and 3309 C grapevine accessions

Abstract

: Deacclimatization results in a loss of bud cold-hardiness whereas cold acclimation results in an increase in it. The regulation of the buds’ cold acclimation and deacclimation in woody plants are poorly understood. This study indicated that differential gene expression during acclimation and transition of the buds from acclimation to deacclimation in the light of dormancy and bud water content. One-year-old vine latent bus of cv. Chambourcin vines and 3309 C ( Vitis riparia x Vitis rupestris ) were used for the analyses. Five genes ( CBF2, CBF4, NCED1, MYB14 , and PLD ) were selected for quantitative real-time PCR. Quantitative real-time PCR was applied to measure gene expression of the dormancy related genes and GAPDH gene was used as a reference gene. CBF2, NCED1 , MYB14, and PLD were strongly upregulated by both with the two-temperature treatment (acclimation (5 °C) and deacclimation (15 °C)) and two different time applications (one and two week) while the expression of CBF4 was low after the applications. Among these four upregulated genes, NCED1 and PLD exhibited the highest level of expression (121.02 and 86.40, respectively) at cold hardy 3309 C during deacclimation (T2, 15 °C). However, for the 5 °C treatment, Chambourcin showed the highest level (32.61) of MYB14 gene expression during T2 application, while 3309 C buds had the highest NCED1 gene (94.83) expression level again during T2 application. According to the results, it is clearly highlighted that PLD and NCED1 gene expression level was highest at cold hardy 3309 C during dormancy and release of dormancy.