Demonstration of fungi in superficial mycosis: Skin scraping and potassium hydroxide mount

Abstract

Skin scraping for demonstration of fungi in superficial mycosis is a commonly performed procedure that aids in the diagnosis of conditions such as dermatophyte infections of the skin and hair, onychomycosis, pityriasis versicolor, and mucocutaneous candidiasis. After taking consent from the patient, specimens are collected onto a black paper and transferred to a glass slide, followed by the addition of potassium hydroxide solution. The slide is examined under the microscope for fungal elements. Careful collection and preparation of specimens are essential for accurate results.