PREPARATION OF SULFATED CHITOSAN/POLYLYSINE/TRANSFORM GROWTH FACTOR-β1 NANOPARTICLES AND THEIR ABILITY TO INDUCE CHONDROGENESIS DIFFERENTIATION OF BMSCs: PREPARATION OF SULFATED CHITOSAN/POLYLYSINE/TRANSFORM GROWTH FACTOR-β1 NANOPARTICLES AND THEIR ABILITY TO INDUCE CHONDROGENESIS DIFFERENTIATION OF
{"title":"PREPARATION OF SULFATED CHITOSAN/POLYLYSINE/TRANSFORM GROWTH FACTOR-β1 NANOPARTICLES AND THEIR ABILITY TO INDUCE CHONDROGENESIS DIFFERENTIATION OF BMSCs: PREPARATION OF SULFATED CHITOSAN/POLYLYSINE/TRANSFORM GROWTH FACTOR-β1 NANOPARTICLES AND THEIR ABILITY TO INDUCE CHONDROGENESIS DIFFERENTIATION OF","authors":"Liu Fei-fei, Wang Qiu-wen, Ma Lie, Gao Chang-you","doi":"10.3724/SP.J.1105.2013.12425","DOIUrl":null,"url":null,"abstract":"采用共沉淀法制备了复合转化生长因子-1(TGF-1)的磺化壳聚糖/聚赖氨酸纳米粒子(SCS/PLL/TGF-1),研究了磺化壳聚糖的浓度对纳米粒子粒径和表面电位的影响.所得纳米微粒的粒径在240~290 nm、表面电位在-0.4~-1.3 mV之间可调.采用透射电子显微镜表征了磺化壳聚糖/聚赖氨酸(SCS/PLL)和SCS/PLL/TGF-1纳米粒子的形貌.通过体外骨髓间充质干细胞(BMSCs)培养实验,分别考察了SCS/PLL/TGF-1纳米粒子对BMSCs的增殖、氨基葡聚糖(GAGs)分泌和Ⅱ型胶原基因表达的影响.结果显示,相对于等量的SCS/PLL或同浓度的自由TGF-1,SCS/PLL/TGF-1纳米粒子可有效保护TGF-1的活性,促进BMSCs向软骨细胞分化,可望应用于软骨修复材料等领域的研究.","PeriodicalId":7133,"journal":{"name":"高分子学报","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2013-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"高分子学报","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.3724/SP.J.1105.2013.12425","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"POLYMER SCIENCE","Score":null,"Total":0}
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