HOMA-IR level in obese type 2 diabetic rat model treated by Sleeve gastrectomy and pancreatic omentoplasty

Abstract

Obesity is a health problem that occurs due to the wrong lifestyle, such as lack of physical activity and the wrong diet. Accumulation of adipose tissue in obesity may increases pro-inflammatory cytokines, particularly in pancreatic beta cells leading to type 2 diabetes mellitus (T2DM). Sleeve gastrectomy (SG) is an alternative therapy for T2DM in obese patients by losing about 40-60% of body weight and increasing GLP-1 secretion which stimulates increased insulin secretion. However, the SG procedure cannot reduce pro-inflammatory cytokines which promote damaged pancreatic cells. Pancreatic omentoplasty can suppress pro-inflammatory cytokines and promote the regeneration of damaged pancreatic cells. The goal of this study is to investigate HOMA-IR in obese rats with diabetes mellitus who underwent SG and pancreatic omentoplasty procedures. An experimental using pre and post-test control group design were done in this study. Eighteen Diabetes Mellitus rats were divided into 3 groups: K1 (SG), K2 (SG + Omentoplasty), and K3 (control). Blood glucose and insulin level were measured using a glucoDR Glucometer Bio-sensor kit and ELISA, respectively before and 10 days after the procedure. HOMA-IR measurement was calculated based on insulin and blood glucose level. A significant decrease of fasting blood gluocese levels were shown in all treatment groups of this study after day 10th. There was a significant increase in the insulin levels after day 10th. Highest decrease of the blood glucose levels and increase of the insulin levels were shown in group K2. Furthermore, a significant decrease of HOMA-IR was shown in the K2 on day 10th. From this study, we may conclude that SG and Pancreas Omentoplasty may significantly reduce the HOMA-IR value in obese rats with T2DM.