{"title":"cDNA Cloning and Molecular Characterization of Mink SMAD4","authors":"Elizabeth Barnes, J. Askham, Pamela F. Jones","doi":"10.1080/10425170290019892","DOIUrl":null,"url":null,"abstract":"The Smad family of proteins have been implicated as major components of the TGF g signalling pathway and are important mediators of its pleiotrophic effects. Here we describe the cloning and characterization of the mink ( Mustela vison ) ortholog of Smad4. Mink Smad4 has a high level of conservation to its human counterpart showing 96% homology at the DNA level and 99% at the amino acid level. This is in agreement with the close homologies seen for the rat and mouse orthologs. In vitro transcription and translation shows the expression of a protein of predicted molecular weight, of identical size to its human counterpart.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"52 1","pages":"47 - 53"},"PeriodicalIF":0.0000,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"DNA Sequence","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/10425170290019892","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
Abstract
The Smad family of proteins have been implicated as major components of the TGF g signalling pathway and are important mediators of its pleiotrophic effects. Here we describe the cloning and characterization of the mink ( Mustela vison ) ortholog of Smad4. Mink Smad4 has a high level of conservation to its human counterpart showing 96% homology at the DNA level and 99% at the amino acid level. This is in agreement with the close homologies seen for the rat and mouse orthologs. In vitro transcription and translation shows the expression of a protein of predicted molecular weight, of identical size to its human counterpart.
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