Detection of chronic obstructive pulmonary disease biomarkers using ROC-analysis

Abstract

Aim. To identify key diagnostic criteria for chronic obstructive pulmonary disease (COPD).Materials and methods. We examined 112 patients with mild, moderate and severe COPD and 32 relatively healthy volunteers with no disturbances in the lung function. The level of IL-4, IL-6, IL-10, IL-17A, TNF-α, IFN-γ, TGF-β1 and CD4+CD126+ cells was determined by means of flow cytometry method. Prooxidant and antioxidant markers of oxidative stress 8-OHdeoxyguanosine, protein carbonyl, glutathione, glutathione reductase, glutathione peroxidase, thioredoxin-1, and thioredoxin reductase-1 were assessed by enzyme immunoassay.Results. The presence of high-ranking correlations of the proinflammatory link of immunity and components of the prooxidant system was revealed, which indicates the presence of systemic alterative processes in patients with COPD. Based on ROC-analysis, key biomarkers were identified in patients with stable COPD: 8-OH-deoxyguanosine, CD4+CD126+ cells, IL-17, and thioredoxin, and their threshold levels were established.Conclusion. The recommended diagnostic criteria will optimize the therapeutic strategies for COPD.