Investigation of The Focusing Effect on The Reconstructed Image Quality in Digital Holographic Microscopy

Abstract

Holography has been used as an imaging method in various fields of science for many years. However, practical applications, challenging procedures and equipment requirements cause restrictions on the use of traditional holography. These requirements and constraints have led to the development of digital holography and digital holographic microscopy. Digital holographic microscopy (DHM) imaging method allows to identify the three dimensional profiles of very small biological samples and transparent objects optically. In digital holographic microscopy, there are substantial advantages in many respects such as obtaining holograms quickly, having complete amplitude and phase information and applying versatile image processing techniques. Although digital holographic microscopy enables the application of many image processing techniques, it is important for the reconstruction process that the recorded image is in the focus of the imaging systems. This paper deals the study of the focusing effects on the reconstructed image obtained from recorded hologram of clear and blurred images with simulation using MATLAB software. The results obtained show that the focusing process in the recording stage is an important factor affecting the construction of holograms and therefore reconstruction performance