Katja Fink, M. Prebil, N. Vardjan, Jørgen Jensen, R. Zorec, M. Kreft
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引用次数: 1
Abstract
Glycogen synthase kinase 3 (GSK-3) plays an important role in metabolic regulation in skeletal muscles, and both insulin and adrenaline stimulate GSK-3 phosphorylation. The aim of the present study was to study the effect of insulin and adrenaline on GSK-3 localisation in skeletal muscles. We characterized subcellular localization of (GSK-3) signal protein in fully differentiated muscle fibre by immunofluorescence and confocal microscopy. We stimulated muscle fibres with insulin and/or adrenaline. Images were analysed by segmentation of single central optical section of the muscle. We found GSK-3 to be localised in clusters. The number of GSK-3 clusters and their average size were increased after stimulation with insulin and/or adrenaline. Average GSK-3 particle size is linearly related to their quantity. We conclude that subcellular GSK-3 in isolated skeletal muscle fibres is localized in clusters and clustering increased after stimulation with insulin and/or adrenaline.
期刊介绍：
Image Analysis and Stereology is the official journal of the International Society for Stereology & Image Analysis. It promotes the exchange of scientific, technical, organizational and other information on the quantitative analysis of data having a geometrical structure, including stereology, differential geometry, image analysis, image processing, mathematical morphology, stochastic geometry, statistics, pattern recognition, and related topics. The fields of application are not restricted and range from biomedicine, materials sciences and physics to geology and geography.
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