Production of myrotoxin B by Myrothecium roridum isolated from Myrothecium leaf spot of mulberry in Japan

R. Murakami, H. Yasui, A. Shirata, A. Kato
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引用次数: 4

Abstract

Myrothecium roridum Tode: Frides, causing leaf spot of mulberry, produces toxins against mulberry. The fungus produces the toxins not only in lesions on mulberry leaves but also on potato sucrose agar medium. Seven toxins, soluble in ethyl acetate, but not in water, were isolated using high pressure liquid chromatograph. Six of the seven toxins were identified with spectroscopic analysis as trichothecenes, including myrotoxin B and D. The remaining toxin was not a trichothecene. The majority (95.1 %) of the isolated toxins was myrotoxin B, which had the highest toxicity against mulberry in the isolated toxins. When spores of M. roridum except for the toxins were used as inoculum on mulberry leaves, no lesions developed. However, the lesion was induced when the spores of M. roridum were inoculated with myrotoxin B at the concentration of which it was not enough to cause necrosis alone. Therefore, myrotoxin B may be an important role in the pathogenicity of M. roridum.
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从日本桑树叶斑病中分离的毒蛾生产毒毒素B
引起桑树叶斑病的毒蛾(Myrothecium roridum Tode)对桑树产生毒素。这种真菌不仅在桑叶上产生毒素,而且在马铃薯蔗糖琼脂培养基上也产生毒素。采用高压液相色谱仪分离了7种可溶于乙酸乙酯而不溶于水的毒素。7种毒素中有6种经光谱分析鉴定为霉菌菌,包括霉菌毒素B和霉菌毒素d,其余毒素不是霉菌菌。其中对桑树毒性最大的毒素为myrotoxin B,占95.1%。除毒素外,以孢子接种桑叶,未发生病变。然而,当毒毒素B单独接种于毒毒素B的浓度不足以引起坏死时,毒毒素B会引起孢子的病变。因此,myrotoxin B可能在毒霉的致病性中起重要作用。
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