Silencing of KCNA1 suppresses the cervical cancer development via mitochondria damage

IF 3.3 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Channels Pub Date : 2019-01-01 DOI:10.1080/19336950.2019.1648627

Li Liu, Yumei Chen, Qingyuan Zhang, Changzhong Li

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引用次数: 17

Abstract

ABSTRACT Voltage-gated potassium channel subfamily A member 1 (KCNA1/Kv1.1) is an important component of type A potassium channels, which has been found to be involved in various tumors. This study aimed to identify the role of KCNA1 in cervical cancer and explore the related mechanism. The levels of KCNA1 in cervical cancer tissues and cell lines were examined by Western blot and qPCR. Cell proliferation and invasion were assessed by CCK-8 and transwell assays, respectively. Protein levels of Hedgehog (Hhg), Wnt and Notch were detected by Western blot. The mitochondrial capacity was examined by immunostaining with MitoTracker Red CMXRos. KCNA1 was highly expressed in cervical cancer tissues and cell lines, and correlated with poor prognosis. In addition, depletion of KCNA1 suppressed growth, proliferation, migration and invasion of HeLa cells. Moreover, KCNA1 could regulate the Hhg, Wnt and Notch signaling pathways and cause mitochondrial dysfunction. The present study has demonstrated that KCNA1 is an oncogene excessively expressed in cervical cancer, and promotes tumor progression by regulating the Hhg, Wnt and Notch signaling pathways and the mitochondrial capacity. Therefore, our results provide a theoretical basis for the discovery of novel clinical treatment against cervical cancer.

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KCNA1的沉默通过线粒体损伤抑制宫颈癌的发展

电压门控钾通道亚家族A成员1 (KCNA1/Kv1.1)是A型钾通道的重要组成部分，已发现其参与多种肿瘤。本研究旨在确定KCNA1在宫颈癌中的作用并探讨其相关机制。Western blot和qPCR检测宫颈癌组织和细胞系中KCNA1的表达水平。分别用CCK-8和transwell检测细胞增殖和侵袭。Western blot检测Hedgehog (Hhg)、Wnt、Notch蛋白水平。用MitoTracker Red CMXRos免疫染色检测线粒体容量。KCNA1在宫颈癌组织和细胞系中高表达，与预后不良相关。此外，KCNA1的缺失抑制HeLa细胞的生长、增殖、迁移和侵袭。此外，KCNA1还能调控Hhg、Wnt和Notch信号通路，导致线粒体功能障碍。本研究表明KCNA1是宫颈癌中过度表达的致癌基因，通过调控Hhg、Wnt、Notch信号通路及线粒体容量促进肿瘤进展。因此，我们的研究结果为发现新的宫颈癌临床治疗方法提供了理论依据。

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来源期刊

Channels 生物-生化与分子生物学

CiteScore

5.90

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Channels is an open access journal for all aspects of ion channel research. The journal publishes high quality papers that shed new light on ion channel and ion transporter/exchanger function, structure, biophysics, pharmacology, and regulation in health and disease. Channels welcomes interdisciplinary approaches that address ion channel physiology in areas such as neuroscience, cardiovascular sciences, cancer research, endocrinology, and gastroenterology. Our aim is to foster communication among the ion channel and transporter communities and facilitate the advancement of the field.