PO-003 Effect of C2C12 Myotubes Function after Electrical Stimulation at Different Time

Abstract

Objective To study the effect of different time of electrical stimulation on C2C12 myotubes function, and further explore its molecular mechanism. Methods An electrical stimulation was given 7 days after C2C12 myotubes differentiation, of which intensity was 30ms, 3Hz, and the stimulation time was 60mins, 120mins, and 180mins, respectively. A total of four experimental groups, including Con (control group), E60 (60 mins group), E120and E180. Microscope was used to observe the muscular myotubes form; Kits were to detect MDA and ROS; Western blot was used to detect the expression of autophagy proteins and mechanism proteins, including PGC1, p-ULK, SIRT1 and SIRT3. Results Compared with the control group, MDA, ROS, SIRT3 increased significantly in E60 (p<0.05), p-ULK and PGC1 increased significantly (p<0.01), SIRT1 decreased significantly (p<0.05). In E120, MDA、ROS、SIRT3 and PGC1 increased significantly (p<0.01), SOD decreased significantly (p<0.05). In E180, MDA and ROS increased significantly (p<0.01), SOD decreased significantly (p<0.01). Conclusions Moderate electrical stimulation can significantly activate oxidative stress, and further promote SIRT3, PGC1 and p-ULK expression, while excessive stimulation has the opposite effects.