Effect of calcium ions on oxygen equilibrium of hemocyanin of asiatic horseshoe crab Tachypleus tridentatus

Comparative biochemistry and physiology Pub Date : 1970-12-15 DOI:10.1016/0010-406X(70)90102-7

Joseph C Hwang , C.P Fung

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引用次数: 13

Abstract

1.
1. The oxygen affinity of the horseshoe crab (Tachypleus tridentatus) hemocyanin is greatly reduced by removing calcium ions either by dialysis against Tris buffer or by addition of EDTA. This effect is largely reversed by the addition of CaCl₂.
2.
2. The logarithmic plot of P₅₀ against calcium ion concentration is a linear function.
3.
3. At low concentrations of calcium ion, the oxygen affinity of hemocyanin varies with changes in the concentration of free calcium ions and also the degree of oxygenation.
4.
4. At concentrations of calcium ion above 20 mM, it depends only on the concentration of free calcium ions.
5.
5. Succinylated hemocyanin has a different absorption spectrum with maximal absorption peaks at 390 mμ. It is incapable of binding reversibly with oxygen.

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钙离子对亚洲马蹄蟹血青素氧平衡的影响

1.1. 马蹄蟹(Tachypleus tridentatus)血青素的氧亲和性通过对Tris缓冲液进行透析或添加EDTA去除钙离子而大大降低。这种效果在很大程度上被CaCl2.2.2的加入所逆转。P50对钙离子浓度的对数曲线为线性函数。在低钙离子浓度下，血青素的氧亲和力随游离钙离子浓度和氧合程度的变化而变化。在钙离子浓度大于20mm时，仅取决于游离钙离子的浓度。琥珀酰化血青素具有不同的吸收光谱，最大吸收峰在390 μ。它不能与氧发生可逆结合。

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Comparative biochemistry and physiology

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