Micro two-dimensional electrophoretic analysis of changes in plasma proteins of next-generation rat of the pregnant rats administered to coplanar PCBs

K. Sakaguchi, J. Suzuki, Masaki Tanaka, M. Shirai, F. Akahori
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引用次数: 1

Abstract

Polychlorinated biphenyl (PCB) 126 or PCB 169 was administered to pregnant rats and kinetic changes in plasma protein spots of their next generation (F2 rats) ages 1, 3, 6, and 15 weeks were observed by micro two-dimensional polyacrylamide gel electrophoresis (M2D-PAGE). In the control group in which age-related changes were observable, changes in the form of the albumin (Alb) spot were recognized at ages 1-3 weeks. Only the Alb spot on the acid side, i.e., non-mercaptoalbumin, was recognized at age 1 week. At age 3 weeks, the Alb spot on the alkaline side, i.e., mercaptoalbumin, also became observable. On the other hand, qualitative changes in the form of Alb, i.e., manifestations of mercaptoalbumin, were recognized at age 1 week by administration of PCB 126. This result suggested that the influence of PCB 126 on the living body is greater than that of PCB 169. In spite of the treatment for toxicity by equivalence of dose control on the basis of toxicity equivalency factor (TEF) 0.1 for PCB 126 (PeCB) and 0.01 for PCB 169 (HxCB), the influence of PCB 126 on the living body was greater than that of PCB 169. The C3 spot was increased at age 3 weeks in the PCB 126 and the PCB 169. The spot showed a tendency toward increase at age 6 weeks in the PCB 126. The TEF compared PCB169 with PCB126, and distinctly increase of the C3 spot was observed for 15 weeks though the TEF was low. From these, as for the PCB 169, it was suggested appearing remarkably in aging influence of the immunity toxicity. The observations of the changes in these spots led to the conclusion that the changes in these spots resulted from transfer of the drugs accumulating in the maternal body to the F2 rats of the maternal rats administered PCB 126 and those administered PCB 169 via the breast milk and that these changes are involved with inflammatory proteins, including the target genes influenced by the toxicity of coplanar PCBs.
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共面多氯联苯对妊娠大鼠下一代血浆蛋白变化的微二维电泳分析
给孕鼠注射多氯联苯(PCB) 126或PCB 169,用微二维聚丙烯酰胺凝胶电泳(M2D-PAGE)观察孕鼠下一代(F2) 1、3、6和15周龄血浆蛋白斑点的动力学变化。在年龄相关变化可观察到的对照组中,白蛋白(Alb)斑点形式的变化在1-3周龄时被识别出来。只有酸性侧的白斑,即非巯基白蛋白,在1周时被识别出来。3周龄时,碱性侧的白斑(即巯基白蛋白)也可观察到。另一方面,白蛋白形式的质变,即巯基白蛋白的表现,在1周时通过给药PCB 126被识别出来。结果表明,PCB 126对生物的影响大于PCB 169。尽管对pc126 (PeCB)和pc169 (HxCB)分别采用毒性等效系数(TEF)为0.1和0.01的剂量控制等效处理,但pc126对生物体的影响大于pc169。3周龄时,PCB 126和PCB 169的C3斑点增加。在PCB 126中,斑点在6周龄时有增加的趋势。将PCB169与PCB126进行TEF比较,虽然TEF较低,但持续15周后C3斑点明显增加。由此可见,多氯联苯169在免疫毒性的老化作用中表现出明显的影响。通过对这些斑点变化的观察,我们得出结论,这些斑点的变化是由于母体体内积累的药物通过母乳转移到给予PCB 126的母体大鼠和给予PCB 169的母体大鼠的F2大鼠,这些变化与炎症蛋白有关,包括受共面PCB毒性影响的靶基因。
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