Cultivation of Schizophyllum commune mushroom on different wood substrates

P. Dasanayaka, S. Wijeyaratne
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引用次数: 10

Abstract

Schizophyllum commune is an edible mushroom grown on wood under natural conditions. Present study focused on cultivation of S.commune on different wood substrates since it is not commercially cultivated. A pure culture of S. commune was obtained by growing a tissue of the mushroom on Potato Dextrose Agar (PDA) medium. Spawns were produced by growing the mycelium on paddy grains. Mushroom was cultivated on sawdust of seven different wood substrates. The maximum yield was observed in sawdust of jackfruit ( Artocarpus heterophyllus ) followed by sawdust of rambutan ( Nephelium lappaceum ) and country almond ( Terminalia catappa ). A significant difference was not observed when mango ( Mangifera indica ) elephant apple ( Dillenia indica ), tulip wood tree ( Harpullia arborea ) and thungfaa ( Alstonia macrophylla ) sawdust used as substrate. The lowest yield was observed in thungfaa ( Alstonia macrophylla ) sawdust. Effect of some additives on the yield was studied and significant difference in yield was observed when rice bran and used-tea leaves used as additives. Effect of rice bran on yield was studied using different ratios of sawdust to rice bran and the highest was observed in 2:1 ratio of sawdust to rice bran. The best incubating temperature for mycelial growth on the substrate was 350C. The composition of the mushroom on a dry weight basis was; 71.4% moisture, 23.35% crude protein and 6% ash. Tested wood species are promising substrates for cultivation of S.communeas cottage industry.
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裂叶菌在不同木质基质上的栽培
裂叶菌(Schizophyllum commune)是一种在自然条件下生长在木头上的食用菌。目前的研究主要集中在不同的木材基质上,因为它不是商业种植的。在马铃薯葡萄糖琼脂(Potato Dextrose Agar, PDA)培养基上培养蘑菇组织,获得了纯菌种。通过在稻谷上生长菌丝来产生卵。在7种不同木材基质的木屑上培养蘑菇。菠萝蜜(Artocarpus heterophyllus)木屑产量最高,其次是红毛丹(Nephelium lappaceum)木屑和山杏仁(Terminalia catappa)木屑。以芒果(Mangifera indica)、象苹果(Dillenia indica)、郁金香木(Harpullia arborea)和大叶木屑为底物时,差异不显著。大叶木屑的产量最低。研究了几种添加剂对产量的影响,以米糠和废茶叶为添加剂,产量差异显著。研究了不同木屑与米糠比例对米糠产量的影响,以2:1的木屑与米糠比例最高。菌丝在培养基上生长的最佳培养温度为350℃。蘑菇的干重组成为;水分71.4%,粗蛋白质23.35%,灰分6%。试验树种是很有前途的青豆家庭手工业栽培基质。
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