Green Synthesis and Characterization of Silver Nanoparticles Using Pterocarpus marsupium and Assessment of its In vitro Antidiabetic Activity

Abstract

Objective: Green synthesis of silver nanoparticles from aqueous extract of Pterocarpus marsupium bark and wood and its characterization. As well as in vitro anti diabetic study was carried out. Method: Synthesis of Pterocarpus marsupium silver nanoparticles was done by drop wise addition of 1 mM silver nitrate to Pterocarpus marsupium bark and wood extract. Characterization of synthesized nanoparticles was carried out by visual examination, UV- Visible absorption spectroscopy, FTIR spectroscopy, Poly dispersity index and Zeta value. In vitro anti diabetic study for the synthesised silver nanoparticles was carried out by α- amylase inhibition assay. Results: The synthesis of silver nanoparticles using Pterocarpus marsupium extract was eco-friendly and cost effective. Aqueous extract of Pterocarpus marsupium bark and wood extract were mixed with the aqueous solution of silver nitrate. The colour of the solution changes from yellow to brown colour due to the surface Plasmon resonance which is the primary confirmation for the formation of silver nanoparticles. The surface Plasmon band in the silver nanoparticles was found to be 431 nm. The FTIR study concluded that hydroxyl and carboxyl groups act as reducing and stabilizing agent and phenolic group function as capping agent. Average particle size was found to be 148.5 nm. It’s polydispersity index was 0.336 and zeta potential was measured and found to be -28 mV with the peak area of 100% intensity. This indicates that formed nanoparticles were stable. The in vitro anti diabetic study confirms that Pterocarpus marsupium is having anti diabetic activity Conclusion: Pterocarpus marsupium silver nanoparticles are found to be effective for anti-diabetic activity. The study found without using any chemical reagent as stabilizing agent the silver nanoparticles are stable. Hence this cost effective and eco-friendly method is highly promising for future.