Evaluation of behavior of human umbilical cord-Wharton’s jelly mesenchymal stem cell on electrospun poly (lactic acid)wax nanofibers scaffold

Abstract

Background: Mesenchymal stem cells are pluripotent stromal cells which are capable of differentiating into different cell lines. Nowadays, umbilical cord Wharton’s jelly (UC-WJ) are increasingly used as sources of stem cells. Studies show that scaffolds can affect the differentiation of stem cells to different cells and cause higher cell viability and proliferation as well. The present study aimed to evaluate the adhesion and viability of WJ-MSCs to PLA/Wax scaffold. Materials and methods: PLA/Wax scaffold was prepared using electrospinning method. Adhesion and viability of MSCs on this scaffold was investigated using scanning electron microscope (SEM) and MTT assay respectively. Results: SEM results showed that the fibers were homogeneous, uniform, and free of beads with high quality property, and adding wax to PLA significantly reduced the diameter of the nanofibers. These studies confirmed that the cells were attached to the scaffold in large numbers and with appropriate size. The results of MTT show good biocompatibility of the scaffold made with the cells and a significant increase in the survival rate of mesenchymal cells was observed during the period. Conclusion: In conclusion, using PLA/Wax scaffold has promoted the attachment, survival and proliferation of the cells and has the potential to be an important candidate for developing the efficiency of 3D-cultures in order to cure diseases.