Multivariate statistical analysis of the essential oils of five Beilschmiediaspecies from Peninsular Malaysia

Wan Mohd Nuzul Hakimi Wan Salleh, S. M. Shaharudin
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Abstract

Identification of the chemical compositionof essential oils is very important for ensuring the quality of finished herbal products. The objective of the study was to analyze the chemical components present in the essential oils of five Beilschmiediaspecies (i.e. B. kunstleri, B. maingayi, B. penangiana, B. madang, and B. glabra) by multivariate data analysis using principal component analysis (PCA) and hierarchical clustering analysis (HCA) methods. The essential oils were obtained by hydrodistillation and fully characterized by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). A total of 108 chemical components were successfully identified from the essential oils of five Beilschmiediaspecies. The essential oils were characterized by high proportions of β-caryophyllene (B.kunstleri), δ-cadinene (B. penangianaand B. madang), and β-eudesmol (B. maingayiand B. glabra). Principal component analysis (PCA) and hierarchical cluster analysis (HCA) revealed that chemical similarity was highest for all samples, except for B. madang. The multivariate data analysis may be used for the identification and characterization of essential oils from different Beilschmiediaspecies that are to be used as raw materials of traditional herbal products.
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马来西亚半岛五种贝氏属植物精油的多元统计分析
精油的化学成分鉴定对保证中药成品的质量非常重要。本研究采用主成分分析(PCA)和层次聚类分析(HCA)方法,对5种白麻属植物(B. kunstleri、B. maingayi、B. penangiana、B. madang和B. glabra)挥发油的化学成分进行分析。采用水蒸气蒸馏法提取精油,并采用气相色谱法(GC)和气相色谱-质谱法(GC- ms)对其进行了表征。从5种贝氏属植物精油中成功鉴定出108种化学成分。其中β-石竹烯(B.kunstleri)、δ-cadinene (B. penangianab . madang)和β- udesmol (B. maingayib . glabra)含量较高。主成分分析(PCA)和层次聚类分析(HCA)表明,除马丹外,所有样品的化学相似性最高。多变量数据分析可用于不同种属植物精油的鉴别和表征,可作为传统草药产品的原料。
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