Fluoxetine induces oxidative stress-dependent DNA damage in human hepatoma cells

IF 0.4 Q4 MEDICINE, RESEARCH & EXPERIMENTAL AIMS Medical Science Pub Date : 2023-01-01 DOI:10.3934/medsci.2023007
Somayeh Boshtam, M. Shokrzadeh, Nasrin Ghassemi-Barghi
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Abstract

Fluoxetine is a selective serotonin reuptake inhibitor that is a commonly used drug for the treatment of depression and obsessive-compulsive disorders. Despite the positive effects of this drug, it seems to be associated with various side effects. Genotoxicity or DNA damage is an important side effect of some kinds of drugs. To date, the genotoxicity and cytotoxicity of fluoxetine are partially unknown. In the present study, some oxidative stress methods were used, such as ROS, MDA and GSH evaluation methods in HepG2 cells treated with fluoxetine (1–10 µM). A comet assay was used to evaluate the genotoxic effects of fluoxetine, and flow cytometry was used for apoptosis detection in these hepatic cells. Our data have shown that fluoxetine increased MDA and intracellular concentration of ROS significantly (P < 0.001), while the amount of GSH was reduced significantly (P < 0.001). Our results also indicated that fluoxetine increased the DNA damage of HepG2 cells. The tail percentage of DNA for control cells was 4%, but this percentage was 19%, 28% and 32% for 1, 5 and 10 µM of fluoxetine concentration, respectively (P < 0.01 and P < 0.001). The flow cytometry results have also shown increases in early and late apoptosis for fluoxetine (13.31% and 9.54%, respectively). In conclusion, the present study has shown that fluoxetine is able to induce oxidative stress-dependent DNA damage. Anyway, more studies are needed to accurately explore the molecular and cellular aspects of fluoxetine.
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氟西汀诱导人肝癌细胞氧化应激依赖性DNA损伤
氟西汀是一种选择性血清素再摄取抑制剂,是一种常用的治疗抑郁症和强迫症的药物。尽管这种药有积极的作用,但它似乎也有各种副作用。遗传毒性或DNA损伤是某些药物的重要副作用。迄今为止,氟西汀的遗传毒性和细胞毒性部分未知。本研究采用氟西汀(1-10µM)处理HepG2细胞后的氧化应激法,如ROS、MDA和GSH评价法。采用彗星试验评估氟西汀的遗传毒性作用,并采用流式细胞术检测肝细胞的凋亡。我们的数据显示,氟西汀显著增加MDA和细胞内ROS浓度(P < 0.001),而显著降低GSH的量(P < 0.001)。氟西汀还增加了HepG2细胞的DNA损伤。对照细胞的DNA尾百分率为4%,而氟西汀浓度为1、5和10µM时的尾百分率分别为19%、28%和32% (P < 0.01和P < 0.001)。流式细胞术结果也显示氟西汀增加了早期和晚期细胞凋亡(分别为13.31%和9.54%)。总之,本研究表明氟西汀能够诱导氧化应激依赖性DNA损伤。无论如何,需要更多的研究来准确地探索氟西汀的分子和细胞方面。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
AIMS Medical Science
AIMS Medical Science MEDICINE, RESEARCH & EXPERIMENTAL-
自引率
14.30%
发文量
20
审稿时长
12 weeks
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