Effects of TiO2 Nanoparticles on Germination and Primary Growth of Mountain Ash (Sorbus luristanica)

Abstract

Introduction: Production of nanoparticles and their use are on the rise in different areas of plant science. However, in spite of their increasing production, there is limited information about their effects on plant biology. In the current study, the potential of TiO 2 nanoparticles was investigated for the purpose of improving seed germination of Sorbus luristanica and then subsequent effects of nanoparticles on the growth and biomass of the plants were determined. Materials and Methods: Seeds of S. luristanica were collected from its natural stands. The seeds were primed with different concentrations of 0, 75, 150, 250, 350 and 500 TiO 2 nanoparticles miligeram per liter for 24 h. The treated seeds were placed in wet sand at room temperature for 2 weeks and then in cold for 3 months. The expriment was set as a completely randimized design with 4 replications. Aftre 3 months of stratification in moistened sand, the stratified seeds were put in the germinator and with the appearance of seed germination signs, germination data were recorded daily during 22 days. At the end of the seed germination experiment, some germination parameters such as seed germination percentage, seed vigority and mean time to germination were calculated. Moreover, some growth and biomass parameters including leaf number, plant height and dry and fresh biomass of leaf, stem as well as roots were measured. In addition, scaning electron microscopic (SEM) was used for observation of presence and adhesiveness of TiO 2 nanoparticles on the seed coat. Results: Based on the results, all the germination parametres including seed germination percentage, seed vigoroty and mean germination time were improved by the TiO 2 nanoparticles treatments. In addition, 500 mg.L -1 treatment considerably improved seed germination characteristics. The peresence of TiO 2 nanoparticles on the treated seeds and lack of the nanomatreials on the conrtol seeds were obsereved by scaning electron microscopic pictures. The One-way ANOVA showed that 75 mg.L -1 treatment was more succesful for improving the grwoth (such as shoot length) and biomass production (fresh and dry biomass of leaf, stem and root and total biomass as well). Conclusion: It can be concluded that priming of the seeds of this species with different concentrations of TiO 2 nanoparticles leads to improvement of seed germination and growth and biomass parameters. However, the patterns of effects were different in each phase. Therefore, the objectives should be formulated first and then the best concentration should be chosen. It seems that with appropriate concentrations, nanoparticles can be useful for breaking seed dormancy and production of the species. Given the promising resutls of 150 mg.L -1 treatment, it can represent a successful treatment for breaking seed dormancy and seedling production of S. luristanica .