Susceptibility of optimization of medium components for the production of antimicrobial secondary metabolites by streptomyces globosus isolated from KAU

A. Baghdadi
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Abstract

The genus Streptomyces represents a group of microorganisms that are widely distributed in nature as the greatest source of the commercially available antibiotics. Objective: In this study, a wide group of actinomycetes strains were isolated from KAU. Further, analysis and evaluate their antibacterial potential against a panel of bacterial pathogens. Methods: In the present study, 12 soil samples were collected from different localities of Al-Baha, Saudi Arabia. Actinomycetes were isolated from these samples using serial dilution and plating method on actinomycetes isolation agar. The antimicrobial activity of actinomycetes was tested against different bacteria. The effect of different media, carbon, nitrogen, and phosphorus sources, on the growth and antibacterial effect against E. coli ATCC35218, E. coli ATCC25922, Salmonella sp., P. aeruginosa ATCC27853, S. aureus ATCCBAA977, and St. pneumonia ATCC49619 were optimized. The results showed that starch-nitrate agar was the best media for the antibacterial activity for S. globosus. The maximum antimicrobial efficacy was produced when S. globosus utilized the starch as a carbon donner. Furthermore, the highest antibacterial activity of S. globosus was created when inorganic nitrogen sources were added, and the best nitrogen source that supported antibacterial production was ammonium sulfate. Di-potassium hydrogen phosphate was the optimum phosphorus sources with 1.5% sodium chloride. Conclusion: Further studies for purification of bioactive metabolites and
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球链霉菌产抗菌次级代谢物培养基组分的敏感性优化
链霉菌属代表了一组广泛分布于自然界的微生物,是商业抗生素的最大来源。目的:从KAU菌株中分离出广泛的放线菌群。进一步,分析和评估其对一组细菌病原体的抗菌潜力。方法:本研究在沙特阿拉伯Al-Baha不同地区采集了12份土壤样品。采用连续稀释法在放线菌分离琼脂上分离放线菌。研究了放线菌对不同细菌的抑菌活性。优化不同培养基、碳源、氮源、磷源对细菌生长及对大肠杆菌ATCC35218、大肠杆菌ATCC25922、沙门氏菌sp.、铜绿假单胞菌ATCC27853、金黄色葡萄球菌ATCCBAA977、肺炎链球菌ATCC49619的抑菌效果的影响。结果表明,淀粉-硝酸盐琼脂培养基对葡萄球菌的抑菌效果最好。当葡萄球菌利用淀粉作为碳受体时,其抗菌效果最大。此外,添加无机氮源时,葡萄球菌的抑菌活性最高,且支持抑菌活性的最佳氮源为硫酸铵。以磷酸氢二钾加1.5%氯化钠为最佳磷源。结论:生物活性代谢物的纯化研究有待进一步深入
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