{"title":"Whole cell electrical impedance spectroscopy for studying ion channel activity","authors":"A. Han, E. Moss, A. Bruno Frazier","doi":"10.1109/SENSOR.2005.1497419","DOIUrl":null,"url":null,"abstract":"In this work, the micro electrophysiological analysis system (/spl mu/-EPAS) is used to study the ion channel activity of single cells. The /spl mu/-EPAS has been designed, fabricated, and characterized for this application. The whole cell analysis system has the capability to perform patch clamping, electrical impedance spectroscopy (EIS), and general extracellular recording using integrated, multi-electrode configurations within a single analysis cavity. Bovine chromaffin cells were used as the target cell to demonstrate and characterize the system. Whole cell impedance measurements were taken over a frequency range of 100 Hz to 5.0 MHz. The impedance signatures of bovine chromaffin cells with: i) the K/sup +/ channels blocked; ii) the Ca/sup 2+/ channels blocked; and iii) unmodified cells were measured and analyzed.","PeriodicalId":22359,"journal":{"name":"The 13th International Conference on Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05.","volume":"51 1","pages":"1704-1707 Vol. 2"},"PeriodicalIF":0.0000,"publicationDate":"2005-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"15","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The 13th International Conference on Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05.","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/SENSOR.2005.1497419","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 15
Abstract
In this work, the micro electrophysiological analysis system (/spl mu/-EPAS) is used to study the ion channel activity of single cells. The /spl mu/-EPAS has been designed, fabricated, and characterized for this application. The whole cell analysis system has the capability to perform patch clamping, electrical impedance spectroscopy (EIS), and general extracellular recording using integrated, multi-electrode configurations within a single analysis cavity. Bovine chromaffin cells were used as the target cell to demonstrate and characterize the system. Whole cell impedance measurements were taken over a frequency range of 100 Hz to 5.0 MHz. The impedance signatures of bovine chromaffin cells with: i) the K/sup +/ channels blocked; ii) the Ca/sup 2+/ channels blocked; and iii) unmodified cells were measured and analyzed.
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