The Dynamic Study in Astrocytes and Cytokines in a Rat Model of Vascular Dementia by Xiongma Dropping Pill

Abstract

OBJECTIVE: To observe the change of Astrocyte (Ast) and cytokines, during vascular dementia (VD) formation to explore the prevention and treatment VD mechanisms of Xiongma dropping pill (XMDP). METHODS: To permanent bilateral ligation of the common carotid artery (2-VO) to establish a VD model. The glial fibrillary acidic protein（GFAP）on the rats brain, and IL-1β, IL-6 and TNF-α were observed by immunohistochemical staining and radioimmunoassay after administration 30 and 60 days. RESULTS: Compared with the sham-operation group, the number of GFAP positive cells and the contents of IL-1β, IL-6 and TNF-α were increased in the model rats (P < 0.05 or P < 0.01). Compared with the model group, XMDP and Ginkgo leaf tablets group could active or proliferative hippocampus and frontal cortex Asts, decrease the number of GFAP positive cells(P < 0.05), and decrease the contents of IL-1β, IL-6 and TNF-α(P < 0.05 or P < 0.01). In addition, the contents of IL-1β, IL-6 and TNF-α in all XMDP groups, after 60 days of administration, were remarkably greater than corresponding concentrations at 30 days(P < 0.05 or P < 0.01). CONCLUSION: XMDP could prevent and treatment VD may be associated with inhibiting the excessive activation or proliferation of Ast and to reduce the generation of the cytokines.