Human BAC Contig Covering the Deleted Region in Pancreatic Cancer at 12q21

E. Youssef, K. Kaneko, T. Yatsuoka, Y. Hayashi, M. Hoshi, A. Horii, T. Furukawa
{"title":"Human BAC Contig Covering the Deleted Region in Pancreatic Cancer at 12q21","authors":"E. Youssef, K. Kaneko, T. Yatsuoka, Y. Hayashi, M. Hoshi, A. Horii, T. Furukawa","doi":"10.3109/10425170109041339","DOIUrl":null,"url":null,"abstract":"In sporadic human primary pancreatic cancer tissues, loss of heterozygosity is frequently observed in the 1-cM region between D12S81 and D12S1719 at 12q21. Loss of this chromosome arm is known to be associate with a poor prognosis in pancreatic cancer patients. Herein we report a complete contig of human bacterial artificial chromosome (BAC) clones covering the deleted region. The region was covered by 21 BAC clones in a minimum tiling path. The clones were confirmed to exist at 12q21 by fluorescence In situ hybridization. We identified novel 40 sequence tagged sites and mapped 10 expressed sequence tags in this region. The BAC contig reported here provides an avenue for determining the complete nucleotide sequence and mining putative tumor suppressor genes in the deleted region of pancreatic cancer at 12q21.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"28 1","pages":"541 - 546"},"PeriodicalIF":0.0000,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"DNA Sequence","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/10425170109041339","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5

Abstract

In sporadic human primary pancreatic cancer tissues, loss of heterozygosity is frequently observed in the 1-cM region between D12S81 and D12S1719 at 12q21. Loss of this chromosome arm is known to be associate with a poor prognosis in pancreatic cancer patients. Herein we report a complete contig of human bacterial artificial chromosome (BAC) clones covering the deleted region. The region was covered by 21 BAC clones in a minimum tiling path. The clones were confirmed to exist at 12q21 by fluorescence In situ hybridization. We identified novel 40 sequence tagged sites and mapped 10 expressed sequence tags in this region. The BAC contig reported here provides an avenue for determining the complete nucleotide sequence and mining putative tumor suppressor genes in the deleted region of pancreatic cancer at 12q21.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
人类BAC序列覆盖胰腺癌12q21缺失区域
在散发性人原发性胰腺癌组织中,在12q21的D12S81和D12S1719之间的1-cM区域经常观察到杂合性缺失。已知这条染色体臂的缺失与胰腺癌患者预后不良有关。在这里,我们报告了一个完整的人类细菌人工染色体(BAC)克隆组覆盖缺失区域。在最小平铺路径上,该区域被21个BAC克隆覆盖。通过荧光原位杂交证实该克隆存在于12q21位点。我们发现了40个新的序列标记位点,并在该区域绘制了10个表达的序列标记。本文报道的BAC序列为确定胰腺癌12q21缺失区域的完整核苷酸序列和挖掘推定的肿瘤抑制基因提供了途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Complete mitochondrial DNA sequence of the yellowfin seabream Acanthopagrus latus and a genomic comparison among closely related sparid species Mitochondrial DNA inaugural issue Comparison of the mitochondrial genomes of East Asian Pseudolabrus fishes Content index A comprehensive analysis of three Asiatic black bear mitochondrial genomes (subspecies ussuricus, formosanus and mupinensis), with emphasis on the complete mtDNA sequence of Ursus thibetanus ussuricus (Ursidae)
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1