Isolation and Screening of Chlorella Sorokiniana for Wastewater Treatment and Biodiesel Production

M. S.S, S. Girisha
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Abstract

- Microalgae grown on wastewater are a probable source of low cost wastewater treatment and biodiesel production. In the study, microalgae was enumerated and identified as C hlorella sorokiniana by 18S rDNA sequence which was cultivated in different wastewater for nutrient removal as well as biodiesel production were studied. The results reveal that the pH of different wastewater samples almost neutralized by microalgae, the total dissolved solids elimination ranging from 52 to 66%, the biological oxygen demand removal efficiency varied much among different wastewaters the removal rate is of 72 to 90%, the chemical oxygen demand removal ranges from 59 to 75%. The magnesium level was decreased and efficiency is about 36 to 60%, the sulphates absorption efficiency was ranging from 57 to 68% and the chloride removal efficiency was 13 to 33%. The lipid content was obtained from the algal biomass which are grown in different wastewater samples was transesterified for biodiesel production, the biodiesel was analyzed by FTIR which meets the ASTM and EU standards, hence from the current study it is evident that Chlorella sorokiniana can be effectively used for potential source for phycoremediation and biodiesel production.
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小球藻的分离与筛选及其废水处理和生物柴油生产
-在废水上生长的微藻是低成本废水处理和生物柴油生产的可能来源。在这项研究中,微藻列举并确认为C hlorella sorokiniana 18 s rDNA序列在不同的养分去除废水培养以及生物柴油的生产进行了研究。结果表明,微藻对不同废水样品的pH值基本中和,对总溶解固体去除率在52% ~ 66%之间,生物需氧量去除率在72% ~ 90%之间,化学需氧量去除率在59% ~ 75%之间。镁含量降低,效率约为36 ~ 60%,硫酸盐吸附效率为57 ~ 68%,氯化物去除效率为13 ~ 33%。通过对不同废水样品中生长的藻类生物质的脂质含量进行酯交换制备生物柴油,并对生物柴油进行FTIR分析,所得生物柴油符合ASTM和EU标准,因此从目前的研究中可以看出,小球藻可以有效地作为生物修复和生物柴油生产的潜在来源。
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