L. Frumin, S. Peltek, S. Bukshpan, V. V. Chasovskikh, G. Zilberstein
{"title":"Anomalous size dependence of the non-linear mobility of DNA","authors":"L. Frumin, S. Peltek, S. Bukshpan, V. V. Chasovskikh, G. Zilberstein","doi":"10.1039/B007756I","DOIUrl":null,"url":null,"abstract":"A strong drift velocity non-linearity of the (doublestranded) DNA molecules vs. the electric field was discovered in gel electrophoresis. The non-linear drift velocity of the longer molecules is higher than that of the short ones, depending on the molecular size in a complex fashion. This behavior contrasts with that of the ordinary, linear, drift velocity where short molecules move faster than the long ones. The molecular size dependence of the non-linear velocity or the non-linear mobility has a non-monotonous wave-like character. The non-linear mobility offers possibilities of manipulating the drift velocity at will?the DNA fragments of different size can be made to move in opposite directions in pulsed field gel electrophoresis","PeriodicalId":20106,"journal":{"name":"PhysChemComm","volume":"72 1","pages":"61-63"},"PeriodicalIF":0.0000,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"PhysChemComm","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1039/B007756I","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
A strong drift velocity non-linearity of the (doublestranded) DNA molecules vs. the electric field was discovered in gel electrophoresis. The non-linear drift velocity of the longer molecules is higher than that of the short ones, depending on the molecular size in a complex fashion. This behavior contrasts with that of the ordinary, linear, drift velocity where short molecules move faster than the long ones. The molecular size dependence of the non-linear velocity or the non-linear mobility has a non-monotonous wave-like character. The non-linear mobility offers possibilities of manipulating the drift velocity at will?the DNA fragments of different size can be made to move in opposite directions in pulsed field gel electrophoresis