Removal of Proteins and Its Effect on Molecular Structure and Properties of Natural Rubber

JST: Engineering and Technology for Sustainable Development Pub Date : 2022-04-15 DOI:10.51316/jst.157.etsd.2022.32.2.2

N. T. Thuong, P. T. Nghia, S. Kawahara

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Abstract

Removal of protein from natural rubber was carried out via deproteinization of natural rubber in the latex stage. Then, its effect on the molecular structure and properties of natural rubber was investigated. Urea, SDS, and acetone were used as denaturing agent, a surfactant, and a polar solvent in the deproteinization, respectively. Various deproteinized natural rubbers were obtained after one, two, and three times of centrifugation with and without acetone, namely DPNR1, DPNR2, DPNR3, DPNR1-A, DPNR2-A, and DPNR3-A. It was found that the total protein content significantly decreased as increasing the number of centrifugations; however, the total fatty acid contents showed a slight decrease. Structural characteristics analyzed by nuclear magnetic resonance spectroscopy indicated no changes in the chemical structure of natural rubber after deproteinization. However, the removal of proteins significantly enhanced the resolution of the NMR signals. Gel content and tensile properties of natural rubber showed a decrease in the removal of proteins, which was associated with the decrease in the number of the inherent branching points formed in natural rubber.

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蛋白质的去除及其对天然橡胶分子结构和性能的影响

通过天然橡胶在乳胶阶段的脱蛋白，实现了天然橡胶中蛋白质的脱除。然后，研究了其对天然橡胶分子结构和性能的影响。尿素、SDS和丙酮分别作为变性剂、表面活性剂和极性溶剂进行脱蛋白。经1、2、3次有丙酮和无丙酮离心得到各种脱蛋白天然橡胶，分别为DPNR1、DPNR2、DPNR3、DPNR1- a、DPNR2- a和DPNR3- a。随着离心次数的增加，总蛋白含量显著降低;但总脂肪酸含量略有下降。核磁共振波谱分析表明，天然橡胶脱蛋白后化学结构没有变化。然而，蛋白质的去除显著提高了核磁共振信号的分辨率。天然橡胶的凝胶含量和拉伸性能显示出蛋白质去除的减少，这与天然橡胶中形成的固有分支点的数量减少有关。

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JST: Engineering and Technology for Sustainable Development

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