{"title":"Evaluation of Lipid Modified Lipase for Interesterification and Hydrolysis Reactions in n-Hexane","authors":"K. D. Green, M. Nakajima, S. Ichikawa, K. Mogi","doi":"10.3136/FSTI9596T9798.3.357","DOIUrl":null,"url":null,"abstract":"Lipase modification by addition of lipid is a simple and effective way to greatly improve enzyme activity for n-hexane based interesterification and hydrolysis reactions. The ranges of modifying lipids and lipases were evaluated, with stearic acid and lipase Saiken 100 (Rhizopus japonicus) investigated in more detail. Enzyme protein recovery and activity were influenced by the quantity of stearic acid addition and the pH of the aqueous preparation phase. Modified lipase protein was characterized using SDS-PAGE electrophoresis. In addition, modified lipase was immobilized within alginate beads allowing for easy biocatalyst separation and re-use for hydrolysis reactions in n-hexane.","PeriodicalId":12457,"journal":{"name":"Food Science and Technology International, Tokyo","volume":"63 1","pages":"357-361"},"PeriodicalIF":0.0000,"publicationDate":"1997-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"10","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Science and Technology International, Tokyo","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3136/FSTI9596T9798.3.357","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 10
Abstract
Lipase modification by addition of lipid is a simple and effective way to greatly improve enzyme activity for n-hexane based interesterification and hydrolysis reactions. The ranges of modifying lipids and lipases were evaluated, with stearic acid and lipase Saiken 100 (Rhizopus japonicus) investigated in more detail. Enzyme protein recovery and activity were influenced by the quantity of stearic acid addition and the pH of the aqueous preparation phase. Modified lipase protein was characterized using SDS-PAGE electrophoresis. In addition, modified lipase was immobilized within alginate beads allowing for easy biocatalyst separation and re-use for hydrolysis reactions in n-hexane.
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