Xingdan Liu, Liping Ouyang, Lan Chen, Yuqin Qiao, Xiaohan Ma, Guohua Xu, Xuanyong Liu
{"title":"Hydroxyapatite composited PEEK with 3D porous surface enhances osteoblast differentiation through mediating NO by macrophage.","authors":"Xingdan Liu, Liping Ouyang, Lan Chen, Yuqin Qiao, Xiaohan Ma, Guohua Xu, Xuanyong Liu","doi":"10.1093/rb/rbab076","DOIUrl":null,"url":null,"abstract":"<p><p>The adverse immune response mediated by macrophages is one of the main factors that are prone to lead poor osseointegration of polyetheretherketone (PEEK) implants in clinic. Hence, endowing PEEK with immunomodulatory ability to avoid the adverse immune response becomes a promising strategy to promote bone repair. In this work, sulfonation and hydrothermal treatment were used to fabricate a 3D porous surface on PEEK and hydroxyapatite (HA) composited PEEK. The HA composited PEEK with 3D porous surface inhibited macrophages polarizing to M1 phenotype and downregulated inducible nitric oxide synthase protein expression, which led to a nitric oxide concentration reduction in culture medium of mouse bone marrow mesenchymal stem cells (mBMSCs) under co-culture condition. The decrease of nitric oxide concentration could help to increase bone formation-related <i>OSX</i> and <i>ALP</i> genes expressions and decrease bone resorption-related <i>MMP-9</i> and <i>MMP-13</i> genes expressions via cAMP-PKA-RUNX2 pathway in mBMSCs. In summary, the HA composited PEEK with 3D porous surface has the potential to promote osteogenesis of PEEK through immunomodulation, which provides a promising strategy to improve the bone repair ability of PEEK.</p>","PeriodicalId":39797,"journal":{"name":"Strategy and Leadership","volume":"40 1","pages":"rbab076"},"PeriodicalIF":0.0000,"publicationDate":"2021-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039504/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Strategy and Leadership","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1093/rb/rbab076","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"Business, Management and Accounting","Score":null,"Total":0}
引用次数: 0
Abstract
The adverse immune response mediated by macrophages is one of the main factors that are prone to lead poor osseointegration of polyetheretherketone (PEEK) implants in clinic. Hence, endowing PEEK with immunomodulatory ability to avoid the adverse immune response becomes a promising strategy to promote bone repair. In this work, sulfonation and hydrothermal treatment were used to fabricate a 3D porous surface on PEEK and hydroxyapatite (HA) composited PEEK. The HA composited PEEK with 3D porous surface inhibited macrophages polarizing to M1 phenotype and downregulated inducible nitric oxide synthase protein expression, which led to a nitric oxide concentration reduction in culture medium of mouse bone marrow mesenchymal stem cells (mBMSCs) under co-culture condition. The decrease of nitric oxide concentration could help to increase bone formation-related OSX and ALP genes expressions and decrease bone resorption-related MMP-9 and MMP-13 genes expressions via cAMP-PKA-RUNX2 pathway in mBMSCs. In summary, the HA composited PEEK with 3D porous surface has the potential to promote osteogenesis of PEEK through immunomodulation, which provides a promising strategy to improve the bone repair ability of PEEK.
期刊介绍：
As a leader responsible for the competitive development of your organization, as well as a desire to keep ahead of the game, you have a responsibility to ensure that you are fully aware of new strategies and developments that can impact upon your personal growth as a leader of others. Strategy & Leadership gives you insight into your own leadership abilities without wasting your time. Each issue brings you informed opinion from leading specialists, gives you professional support and looks at strategies, tools and techniques from an objective position. Strategy & Leadership explains the how and why in order to keep you clearly focused. Its thoughtful, in-depth analysis and advice tells you where you should be going and what to avoid on your journey. Strategy & Leadership is a bi-monthly briefing that brings you the thoughts, advice and analysis of some of the world''s current revolutionary thinkers. Your subscription will show you: -How to improve existing leadership skills and develop new skills -Maintain a competitive position for your organization -Motivate your workforce -Adapt strategies in the light of technological change -Reassess current strategies based on practical insights into how other organizations have structured and managed change for a success -Consider new theories that could reshape current thinking and create future success -Benefit from the advice of prominent futurists -Learn from the experiences of leading CEOs and avoid the pitfalls that they have encountered -Select, develop and retain senior colleagues who will help you steer your organization in the future -Stay one step ahead of the competition - both internally and externally -Gain an insight into competitors'' strategies -Keep abreast of the latest trends in strategic planning -Implement successful strategies for your organization
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
