Technology of obtaining and chemical profiles of dry extracts from leaves of prospective species of the hawthorn genus

Q3 Pharmacology, Toxicology and Pharmaceutics ScienceRise: Pharmaceutical Science Pub Date : 2023-02-28 DOI:10.15587/2519-4852.2023.274772

N. Sydora, V. Yakovenko, O. Ochkur, A. Rudnyk, O. Honcharov, Svetlana Zuikina, K. Romas, M. Marchenko

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Abstract

The aim of the work was to develop a technology for obtaining dry extracts from leaves of non-pharmacopoeia species of hawthorn and establishing their chemical profiles. Materials and methods. Leaves of Crataegus mollis Sarg., Crataegus submollis Sarg. and Crataegus arnoldiana Sarg., used to obtain the dry extracts, were harvested in the Botanical Garden of the National University named after M. N. Karazina in August 2021. To determine the chemical profiles of the obtained dry extracts, the spectrophotometric method and the high-performance liquid chromatography (HPLC) method were used. Results. A technological scheme for obtaining dry extracts of hawthorn leaves has been developed. The content of flavonoids and hydroxycinnamic acids in dry extracts of the leaves of C. mollis Sarg., C. submollis Sarg. and C. arnoldiana Sarg. was determined. The content of free amino acids in extracts ranges from 0.70 % to 0.99 %. The content of flavonoids ranged from 7.25 %±0.04 to 8.43 %±0.01, of hydroxycinnamic acids - from 2.35 %±0.02 to 2.85 %±0.03. Rutin, kaempferol-3-O-glycoside, epicatechin and chlorogenic acid were identified in all extracts by the HPLC method. In dry leaf extracts of C. arnoldiana Sarg. and C. mollis identified p-coumaric acid; C. arnoldiana Sarg. – quercetin; C. arnoldiana Sarg. and C. submollis Sarg. – ferulic acid; C. submollis Sarg. and C. mollis Sarg. – quercetin-3-O-rhamnoside. Conclusions. Dry leaf extracts of C. mollis Sarg., C. submollis Sarg. and C. arnoldiana Sarg. were obtained. For the first time, the content of flavonoids and hydroxycinnamic acids in the extracts was determined by HPLC. It was established that the chemical profile of the obtained extracts is formed by rutin, chlorogenic acid and kaempferol-3-O-glycoside, which can be used in further standardization of these substances. In addition, a comparative study of the amino acid composition of the obtained extracts was carried out for the first time

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山楂属潜在植物叶片干提取物提取技术及化学成分分析

本研究的目的是开发一种从非药典物种山楂叶中提取干提取物的技术，并建立其化学图谱。材料和方法。山楂叶。亚molis cretaus Sarg;和山楂。用于提取干提取物的植物于2021年8月在以M. N. Karazina命名的国立大学植物园收获。采用分光光度法和高效液相色谱法测定了所制干提取物的化学成分。提出了一种提取山楂叶干提取物的工艺方案。研究了肉桂叶干提取物中黄酮类化合物和羟基肉桂酸的含量。， C. submollis Sarg。和C. arnoldiana Sarg。是确定。提取物中游离氨基酸含量为0.70% ~ 0.99%。黄酮类化合物含量为7.25%±0.04 ~ 8.43%±0.01，羟基肉桂酸含量为2.35%±0.02 ~ 2.85%±0.03。HPLC法鉴定出芦丁、山奈酚-3- o -糖苷、表儿茶素和绿原酸。野车前草干叶提取物。C. mollis鉴定对香豆酸;C. arnoldiana;——槲皮素;C. arnoldiana;和C. submollis Sarg。-阿魏酸;亚mollis;C. mollis Sarg。——quercetin-3-O-rhamnoside.Conclusions。黄芩干叶提取物。， C. submollis Sarg。和C. arnoldiana Sarg。被获得。首次采用高效液相色谱法测定了黄酮和羟基肉桂酸的含量。确定所得提取物的化学谱由芦丁、绿原酸和山奈酚-3- o -糖苷组成，可用于这些物质的进一步标准化。此外，还首次对所得提取物的氨基酸组成进行了比较研究

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