{"title":"Comparison of a toxin neutralization assay and ELISA for determination of pertussis toxin antibodies","authors":"Jerker Isacson , Birger Trollfors , Teresa Lagergrd , John Taranger","doi":"10.1016/S0888-0786(96)01072-4","DOIUrl":null,"url":null,"abstract":"<div><p>Sera from 192 children participating in studies of a pertussis toxoid vaccine were analyzed by enzyme-linked immunosorbent assay (ELISA) for pertussis toxin IgG antibodies and by the Chinese hamster ovary cell assay for pertussis toxin neutralizing antibodies. A significant linear correlation was found between titers obtained by ELISA and the neutralization assay (<em>r</em> = 0.84, <em>P</em> < 0.0001). The study shows that these two assays give similar information for groups of sera, although an antibody titer obtained by one method could generally not be used to predict a titer for the other method with accuracy in an individual serum. We propose that ELISA alone can be used in studies of IgG antibody response to pertussis toxin after vaccination with pertussis toxoid.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"8 3","pages":"Pages 163-167"},"PeriodicalIF":0.0000,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0888-0786(96)01072-4","citationCount":"6","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Serodiagnosis and Immunotherapy in Infectious Disease","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0888078696010724","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 6
Abstract
Sera from 192 children participating in studies of a pertussis toxoid vaccine were analyzed by enzyme-linked immunosorbent assay (ELISA) for pertussis toxin IgG antibodies and by the Chinese hamster ovary cell assay for pertussis toxin neutralizing antibodies. A significant linear correlation was found between titers obtained by ELISA and the neutralization assay (r = 0.84, P < 0.0001). The study shows that these two assays give similar information for groups of sera, although an antibody titer obtained by one method could generally not be used to predict a titer for the other method with accuracy in an individual serum. We propose that ELISA alone can be used in studies of IgG antibody response to pertussis toxin after vaccination with pertussis toxoid.
对参加百日咳类毒素疫苗研究的192例儿童血清进行百日咳毒素IgG抗体酶联免疫吸附试验(ELISA)和中国仓鼠卵巢细胞法百日咳毒素中和抗体检测。ELISA测定的滴度与中和试验结果呈显著的线性相关(r = 0.84, P <0.0001)。研究表明，这两种方法对血清组给出了相似的信息，尽管用一种方法获得的抗体滴度通常不能准确地用于预测另一种方法在单个血清中的滴度。我们建议单独使用ELISA法研究百日咳类毒素疫苗接种后IgG抗体对百日咳毒素的反应。
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