{"title":"Detection of Acidovorax avenae subsp. citrulli using PCR and MALDI-TOF MS","authors":"H. Kajiwara, Masatoshi Sato, A. Suzuki","doi":"10.2198/JELECTROPH.56.13","DOIUrl":null,"url":null,"abstract":"Virulent strains of Acidovorax avenae subsp. citrulli (Aac) and avenae cause bacterial fruit blotch in cucurbits and bacterial stripe in rice, respectively. Here, we describe a rapid (1 h) method to identify virulent strains of Aac using a combination of short PCR and matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Single-stranded DNA of the PCR products was analyzed by MALDI-TOF MS after digestion by an enzyme and alkali denaturation. The mass spectra differed among the strains as a result of the differences in their nucleotide sequences. This new method allows rapid identification of virulent strains of Aac.","PeriodicalId":15059,"journal":{"name":"Journal of capillary electrophoresis","volume":"108 1","pages":"13-17"},"PeriodicalIF":0.0000,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"9","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of capillary electrophoresis","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2198/JELECTROPH.56.13","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 9
Abstract
Virulent strains of Acidovorax avenae subsp. citrulli (Aac) and avenae cause bacterial fruit blotch in cucurbits and bacterial stripe in rice, respectively. Here, we describe a rapid (1 h) method to identify virulent strains of Aac using a combination of short PCR and matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Single-stranded DNA of the PCR products was analyzed by MALDI-TOF MS after digestion by an enzyme and alkali denaturation. The mass spectra differed among the strains as a result of the differences in their nucleotide sequences. This new method allows rapid identification of virulent strains of Aac.