Pulsed Low Intensity Electromagnetic Field (PEMF) Affects Cell Cycle of Human Osteoblast-like Cells in Vi tro

N. Rosenberg, O. Rosenberg, M. Soudry
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引用次数: 8

Abstract

High frequency electro magnetic field in the range of 4 - 33 kHz given in low frequency bursts of 7-15 Hz (PEMF) is efficient in stimulating osteoblast proliferation and differentiation in vitro. We hypothesize that osteoblast cell cycle changes following this stimulatory PEMF. In order to explore this effect we used primary explant cultures of human osteoblast-like cells. These cells were subjected to low frequency of 20-30 Hz, h igher frequency of 5-15 kHz electro magnetic fields and to PEM F (5-15 kHz basic frequency in pulses of 20-30 Hz) with maximal magnetic flu x of 10-12 Gauss. The maturation state of the cells was estimated by the measurement of cellular alkaline phosphatase activity and the overall cell death by the LDH activity in culture media. The cell cycle was analyzed by cytometry. The PEMF stimulation significantly increased the proportion of cells in the G1 phase (p<.001) and decreased the proportion of apoptotic and necrotic cells (p<.001), with parallel decrease in cellular alkaline phosphatase and media LDH act ivities. Pure 20-30 Hz or 5-15 kHz electro magnetic stimu lations had no effect on these parameters. Therefore we show that PEM F with a basic frequency in the kHz range provided in pulses in the infrasonic range alters human osteoblast cell cycle by reducing cell death and cellular maturation.
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脉冲低强度电磁场对体外人成骨样细胞周期的影响
在4 ~ 33khz范围内,以7 ~ 15hz的低频脉冲(PEMF)给予高频电磁场,可有效刺激体外成骨细胞的增殖和分化。我们假设成骨细胞周期在这种刺激的PEMF后发生变化。为了探索这种影响,我们使用了人成骨样细胞的原代外植体培养。这些细胞受到低频20-30赫兹、高频5-15千赫的电磁场和PEM F(20-30赫兹脉冲中5-15千赫的基本频率)的最大磁流x为10-12高斯。通过测定细胞碱性磷酸酶活性来判断细胞的成熟状态,通过培养基中LDH活性来判断细胞的整体死亡情况。用细胞术分析细胞周期。PEMF刺激显著增加了G1期细胞的比例(p< 0.001),降低了凋亡和坏死细胞的比例(p< 0.001),同时细胞碱性磷酸酶和培养基LDH活性也显著降低。单纯的20- 30hz或5- 15khz电磁刺激对这些参数没有影响。因此,我们表明,在次声范围内的脉冲中提供的基频在kHz范围内的PEM F通过减少细胞死亡和细胞成熟来改变人类成骨细胞周期。
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