The Effect of Metformin Levels on Ovarian Activity, Embryo Quality and Reproductive Performance of (Baladi Black) Rabbit Does

Abstract

This education was conducted toward investigate the effect of metformin on ovarian activity and embryo quality of local breed rabbit does. Atotal of 21 mature does were alienated into three groups (7 rabbits in each). Does in the 1group were assumed oral 3ml sterilized saline (Control, G1). Does in the 2 (G2) and 3 (G3)groups were specified 20 and 40 mg metforminm mg /kglive body weight , respectively for 3 weeks before neutral coupling by fertile bucks from the matching breed. Weight and litter size at natal and weaning were documented from 4 does in each group.Embryos being composed by flushing from 3 does in each group with phosphate buffer solution from oviduct and uterine horn in each doe in all groups after 72 hrs of mating, where the ovaries structure on the ovarian site was recorded. Inconsequential contrasts between location of the ovary within the cruel number of little, medium and large follicles. Whereas, the cruel number of CL and bleeding follicles in right were altogether (P<0.05) higher than cleared out (5.78 vs. 4.11) and (1.22 vs. 0.67). The rate of practical embryos was higher in G1 (95.2%, P<0.05) than those in G2 (88.9%) and in G3 (75.0%). Whereas fetus recuperation rate was higher in G2 than in other bunches, but the adjustments among them were not noteworthy. These results indicated that treatment improved ovarian response and reproductive performance although it's reducing embryo recovery and viability rate for rabbit does.