The Arabidopsis GRAS Protein SCL14 Interacts with Class II TGA Transcription Factors and Is Essential for the Activation of Stress-Inducible Promoters[C][W]

Benjamin Fode, Tanja Siemsen, Corinna Thurow, R. Weigel, C. Gatz
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引用次数: 222

Abstract

The plant signaling molecule salicylic acid (SA) and/or xenobiotic chemicals like the auxin mimic 2,4-D induce transcriptional activation of defense- and stress-related genes that contain activation sequence-1 (as-1)–like cis-elements in their promoters. as-1–like sequences are recognized by basic/leucine zipper transcription factors of the TGA family. Expression of genes related to the SA-dependent defense program systemic acquired resistance requires the TGA-interacting protein NPR1. However, a number of as-1–containing promoters can be activated independently from NPR1. Here, we report the identification of Arabidopsis thaliana SCARECROW-like 14 (SCL14), a member of the GRAS family of regulatory proteins, as a TGA-interacting protein that is required for the activation of TGA-dependent but NPR1-independent SA- and 2,4-D–inducible promoters. Chromatin immunoprecipitation experiments revealed that class II TGA factors TGA2, TGA5, and/or TGA6 are needed to recruit SCL14 to promoters of selected SCL14 target genes identified by whole-genome transcript profiling experiments. The coding regions and the expression profiles of the SCL14-dependent genes imply that they might be involved in the detoxification of xenobiotics and possibly endogenous harmful metabolites. Consistently, plants ectopically expressing SCL14 showed increased tolerance to toxic doses of the chemicals isonicotinic acid and 2,4,6-triiodobenzoic acid, whereas the scl14 and the tga2 tga5 tga6 mutants were more susceptible. Hence, the TGA/SCL14 complex seems to be involved in the activation of a general broad-spectrum detoxification network upon challenge of plants with xenobiotics.
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拟南芥GRAS蛋白SCL14与ⅱ类TGA转录因子相互作用的研究[C][W]
植物信号分子水杨酸(SA)和/或生长素模拟物2,4- d等外源化学物质诱导防御和应激相关基因的转录激活,这些基因在其启动子中含有激活序列-1 (as-1)样顺式元件。as-1-like序列被TGA家族的基本/亮氨酸拉链转录因子识别。sa依赖性防御程序系统获得性耐药相关基因的表达需要tga相互作用蛋白NPR1。然而,许多含有as-1的启动子可以独立于NPR1被激活。在这里,我们报道了GRAS调节蛋白家族成员Arabidopsis thaliana稻草人样14 (SCL14)作为tga相互作用蛋白的鉴定,该蛋白是激活tga依赖但不依赖npr1的SA-和2,4- d诱导启动子所必需的。染色质免疫沉淀实验显示,II类TGA因子TGA2、TGA5和/或TGA6需要将SCL14招募到由全基因组转录谱分析实验鉴定的选定SCL14靶基因的启动子中。scl14依赖基因的编码区和表达谱表明,它们可能参与了对外源物和内源性有害代谢物的解毒。与此一致的是,异位表达SCL14的植株对异烟酸和2,4,6-三碘苯甲酸的耐受性增强,而SCL14和tga2 tga5 tga6突变体更敏感。因此,TGA/SCL14复合体似乎参与了植物受到外源物质攻击时广谱解毒网络的激活。
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