{"title":"Visualization of replicating bands in plant chromosomes with a monoclonal anti-BrdU antibody method","authors":"K. Taniguchi, R. Tanaka","doi":"10.1266/JJG.66.485","DOIUrl":null,"url":null,"abstract":"In order to detect DNA replication in chromosomes of four plant species, Allium fistulosum (2n=16), Crepis capillaris (2n=6), Haplopappus gracilis (2n=4) and Chrysanthemum coronarium var. spatiosum (2n=18), an immunological technique using monoclonal anti-BrdU antibody was utilized. In this technique, HCl was used for denaturation and avidin-biotin complex for the immunological process. The replicating bands which appeared with this technique were of unusually high resolution.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"27 1","pages":"485-489"},"PeriodicalIF":0.0000,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"7","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Japanese Journal of Genetics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1266/JJG.66.485","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 7
Abstract
In order to detect DNA replication in chromosomes of four plant species, Allium fistulosum (2n=16), Crepis capillaris (2n=6), Haplopappus gracilis (2n=4) and Chrysanthemum coronarium var. spatiosum (2n=18), an immunological technique using monoclonal anti-BrdU antibody was utilized. In this technique, HCl was used for denaturation and avidin-biotin complex for the immunological process. The replicating bands which appeared with this technique were of unusually high resolution.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
